TY - JOUR
T1 - Cyclic AMP-dependent phosphorylation of a brain inositol trisphosphate receptor decreases its release of calcium
AU - Supattapone, S.
AU - Danoff, S. K.
AU - Theibert, A.
AU - Joseph, S. K.
AU - Steiner, Joseph P.
AU - Snyder, S. H.
PY - 1988
Y1 - 1988
N2 - We report the stoichiometric phosphorylation of an inositol 1,4,5-trisphosphate receptor-binding protein from rat brain by the cAMP-dependent protein kinase but not by protein kinase C or Ca2+/calmodulin-dependent protein kinase. This phosphorylation event does not markedly alter [3H]inositol 1,4,5-trisphosphate-binding characteristics. However, inositol 1,4,5-trisphosphate is only 10% as potent in releasing 45Ca2+ from phosphorylated, as compared with native, cerebellar microsomes. Phosphorylation of the inositol 1,4,5-trisphosphate-binding protein by the cAMP-dependent protein kinase may provide a biochemical substrate for second-messenger cross talk.
AB - We report the stoichiometric phosphorylation of an inositol 1,4,5-trisphosphate receptor-binding protein from rat brain by the cAMP-dependent protein kinase but not by protein kinase C or Ca2+/calmodulin-dependent protein kinase. This phosphorylation event does not markedly alter [3H]inositol 1,4,5-trisphosphate-binding characteristics. However, inositol 1,4,5-trisphosphate is only 10% as potent in releasing 45Ca2+ from phosphorylated, as compared with native, cerebellar microsomes. Phosphorylation of the inositol 1,4,5-trisphosphate-binding protein by the cAMP-dependent protein kinase may provide a biochemical substrate for second-messenger cross talk.
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U2 - 10.1073/pnas.85.22.8747
DO - 10.1073/pnas.85.22.8747
M3 - Article
C2 - 2847175
AN - SCOPUS:0000559009
SN - 0027-8424
VL - 85
SP - 8747
EP - 8750
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -