TY - JOUR
T1 - Cyclic AMP-dependent control of the rat hepatic glutathione disulfide-sulfhydryl ratio
AU - Isaacs, John T.
AU - Binkley, Francis
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1977/6/23
Y1 - 1977/6/23
N2 - The disulfide-sulfhydryl ratio of rat hepatic tissue has been found to vary diurnally lowest in the early morning and highest in the early evening (Isaacs, J. (1976) Fed. Proc. 35, 1472, and Isaacs J. and Binkley, F. (1977) Biochim. Biophys. Acta 497, 192-204). Intraperitoneal injections of dibutyryl cyclic AMP induces an increase in hepatic glutathione protein mixed disulfides (GSSProt) combined with a corresponding decrease in reduced glutathione (GSH) and proteon sulfhydryl (ProtSH). Also, dibutyryl cyclic AMP caused hepatic catalase activity to decrease and to increase hepatic production of peroxide molecules. A decrease in catalase activity directs more of the increased peroxide into the glutathione peroxidase pathway. This leads to increased amounts of oxidized glutathione (GSSG) which ultimately results in increased levels of GSSProt. Therefore cyclic AMP may mediate its effect on the disulfide-sulfhydryl ratio via control over catalse and peroxidase generation. Support for this idea is provided by the close temporal correlation between the diurnal variations in cyclic AMP, hepatic catalase, peroxidase generation and GSSProt-GSH levels.
AB - The disulfide-sulfhydryl ratio of rat hepatic tissue has been found to vary diurnally lowest in the early morning and highest in the early evening (Isaacs, J. (1976) Fed. Proc. 35, 1472, and Isaacs J. and Binkley, F. (1977) Biochim. Biophys. Acta 497, 192-204). Intraperitoneal injections of dibutyryl cyclic AMP induces an increase in hepatic glutathione protein mixed disulfides (GSSProt) combined with a corresponding decrease in reduced glutathione (GSH) and proteon sulfhydryl (ProtSH). Also, dibutyryl cyclic AMP caused hepatic catalase activity to decrease and to increase hepatic production of peroxide molecules. A decrease in catalase activity directs more of the increased peroxide into the glutathione peroxidase pathway. This leads to increased amounts of oxidized glutathione (GSSG) which ultimately results in increased levels of GSSProt. Therefore cyclic AMP may mediate its effect on the disulfide-sulfhydryl ratio via control over catalse and peroxidase generation. Support for this idea is provided by the close temporal correlation between the diurnal variations in cyclic AMP, hepatic catalase, peroxidase generation and GSSProt-GSH levels.
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U2 - 10.1016/0304-4165(77)90084-8
DO - 10.1016/0304-4165(77)90084-8
M3 - Article
C2 - 18207
AN - SCOPUS:0017380975
VL - 498
SP - 29
EP - 38
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 1
ER -