Abstract
Using a modified Boyden chamber assay, we have examined the effect of cyclic nucleotides on bovine aortic endothelial cell migration in vitro. Dibutyrl cyclic 3′,5′-adenosine monophosphate (5mM) inhibited endothelial cell random migration by 67% and inhibited fibronectin-induced chemotaxis by 75%. Agents which signicantly stimulated adenylate cyclase activity in endothelial cell membranes were also effective inhibitors of endothelial cell migration. Timolol blocked both the isoproterenol-induced stimulation of adenylate cyclase and the ability of isoproterenol to inhibit endothelial cell migration. Caffeine and isoproterenol together had a greater inhibitory effect on endothelial cell motility than either alone. These data suggest that cAMP may modulate vascular endothelial cell migration in an inhibitory fashion.
Original language | English (US) |
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Pages (from-to) | 279-287 |
Number of pages | 9 |
Journal | Cell Biology International Reports |
Volume | 11 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology