Cyclic 3′,5′-adenosine monophosphate modulates vascular endothelial cell migration in vitro

Sean F. Hackett, Zvi Friedman, Peter A. Campochiaro

Research output: Contribution to journalArticle

Abstract

Using a modified Boyden chamber assay, we have examined the effect of cyclic nucleotides on bovine aortic endothelial cell migration in vitro. Dibutyrl cyclic 3′,5′-adenosine monophosphate (5mM) inhibited endothelial cell random migration by 67% and inhibited fibronectin-induced chemotaxis by 75%. Agents which signicantly stimulated adenylate cyclase activity in endothelial cell membranes were also effective inhibitors of endothelial cell migration. Timolol blocked both the isoproterenol-induced stimulation of adenylate cyclase and the ability of isoproterenol to inhibit endothelial cell migration. Caffeine and isoproterenol together had a greater inhibitory effect on endothelial cell motility than either alone. These data suggest that cAMP may modulate vascular endothelial cell migration in an inhibitory fashion.

Original languageEnglish (US)
Pages (from-to)279-287
Number of pages9
JournalCell Biology International Reports
Volume11
Issue number4
DOIs
StatePublished - Apr 1987
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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