CXCR4 regulates Plasmodium development in mouse and human hepatocytes

Hironori Bando, Ariel Pradipta, Shiroh Iwanaga, Toru Okamoto, Daisuke Okuzaki, Shun Tanaka, Joel Vega-Rodríguez, Youngae Lee, Ji Su Ma, Naoya Sakaguchi, Akira Soga, Shinya Fukumoto, Miwa Sasai, Yoshiharu Matsuura, Masao Yuda, Marcelo Jacobs-Lorena, Masahiro Yamamoto

Research output: Contribution to journalArticle

Abstract

The liver stage of the etiological agent of malaria, Plasmodium, is obligatory for successful infection of its various mammalian hosts. Differentiation of the rod-shaped sporozoites of Plasmodium into spherical exoerythrocytic forms (EEFs) via bulbous expansion is essential for parasite development in the liver. However, little is known about the host factors regulating the morphological transformation of Plasmodium sporozoites in this organ. Here, we show that sporozoite differentiation into EEFs in the liver involves protein kinase C ζ-mediated NF-κB activation, which robustly induces the expression of C-X-C chemokine receptor type 4 (CXCR4) in hepatocytes and subsequently elevates intracellular Ca2+ levels, thereby triggering sporozoite transformation into EEFs. Blocking CXCR4 expression by genetic or pharmacological intervention profoundly inhibited the liver-stage development of the Plasmodium berghei rodent malaria parasite and the human Plasmodium falciparum parasite. Collectively, our experiments show that CXCR4 is a key host factor for Plasmodium development in the liver, and CXCR4 warrants further investigation for malaria prophylaxis.

Original languageEnglish (US)
Pages (from-to)1733-1748
Number of pages16
JournalThe Journal of experimental medicine
Volume216
Issue number8
DOIs
StatePublished - Aug 5 2019

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CXC Chemokines
Plasmodium
Chemokine Receptors
Sporozoites
Hepatocytes
Liver
Parasites
Malaria
Plasmodium malariae
Plasmodium berghei
Plasmodium falciparum
Protein Kinase C
Rodentia
Pharmacology
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Bando, H., Pradipta, A., Iwanaga, S., Okamoto, T., Okuzaki, D., Tanaka, S., ... Yamamoto, M. (2019). CXCR4 regulates Plasmodium development in mouse and human hepatocytes. The Journal of experimental medicine, 216(8), 1733-1748. https://doi.org/10.1084/jem.20182227

CXCR4 regulates Plasmodium development in mouse and human hepatocytes. / Bando, Hironori; Pradipta, Ariel; Iwanaga, Shiroh; Okamoto, Toru; Okuzaki, Daisuke; Tanaka, Shun; Vega-Rodríguez, Joel; Lee, Youngae; Ma, Ji Su; Sakaguchi, Naoya; Soga, Akira; Fukumoto, Shinya; Sasai, Miwa; Matsuura, Yoshiharu; Yuda, Masao; Jacobs-Lorena, Marcelo; Yamamoto, Masahiro.

In: The Journal of experimental medicine, Vol. 216, No. 8, 05.08.2019, p. 1733-1748.

Research output: Contribution to journalArticle

Bando, H, Pradipta, A, Iwanaga, S, Okamoto, T, Okuzaki, D, Tanaka, S, Vega-Rodríguez, J, Lee, Y, Ma, JS, Sakaguchi, N, Soga, A, Fukumoto, S, Sasai, M, Matsuura, Y, Yuda, M, Jacobs-Lorena, M & Yamamoto, M 2019, 'CXCR4 regulates Plasmodium development in mouse and human hepatocytes', The Journal of experimental medicine, vol. 216, no. 8, pp. 1733-1748. https://doi.org/10.1084/jem.20182227
Bando H, Pradipta A, Iwanaga S, Okamoto T, Okuzaki D, Tanaka S et al. CXCR4 regulates Plasmodium development in mouse and human hepatocytes. The Journal of experimental medicine. 2019 Aug 5;216(8):1733-1748. https://doi.org/10.1084/jem.20182227
Bando, Hironori ; Pradipta, Ariel ; Iwanaga, Shiroh ; Okamoto, Toru ; Okuzaki, Daisuke ; Tanaka, Shun ; Vega-Rodríguez, Joel ; Lee, Youngae ; Ma, Ji Su ; Sakaguchi, Naoya ; Soga, Akira ; Fukumoto, Shinya ; Sasai, Miwa ; Matsuura, Yoshiharu ; Yuda, Masao ; Jacobs-Lorena, Marcelo ; Yamamoto, Masahiro. / CXCR4 regulates Plasmodium development in mouse and human hepatocytes. In: The Journal of experimental medicine. 2019 ; Vol. 216, No. 8. pp. 1733-1748.
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