TY - JOUR
T1 - CXCR3 marks CD4+ memory T lymphocytes that are competent to migrate across a human brain microvascular endothelial cell layer
AU - Callahan, Melissa K.
AU - Williams, Katherine A.
AU - Kivisäkk, Pia
AU - Pearce, Donna
AU - Stins, Monique F.
AU - Ransohoff, Richard M.
N1 - Funding Information:
This investigation was supported in part by Postdoctoral Fellowship FG1482-A-1 from the National Multiple Sclerosis Society (M.K.C.) and by PO1 NS38667 (R.M.R.).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/8
Y1 - 2004/8
N2 - Chemokines and their receptors may be implicated in leukocyte ingress into brain during inflammation observed during the course of multiple sclerosis (MS). To address receptor modulation on CD4+ memory T lymphocytes during diapedesis, we used an in vitro model of the blood-brain barrier (BBB). We found that only memory (CD45RO+) cells transmigrated and type 3 CXC chemokine receptor (CXCR3) was enriched on transmigrated cells. CXCR3 depletion of the input population did not affect transmigration capability. CXCR3 reemerged on CXCR3 depleted cells independently of endothelial cell exposure, but was susceptible to incubation at 4°C, indicating receptor recycling. We propose that CXCR3 serves as a surface marker for cells that have the capacity to cross the BBB, but does not play an essential role in extravasation.
AB - Chemokines and their receptors may be implicated in leukocyte ingress into brain during inflammation observed during the course of multiple sclerosis (MS). To address receptor modulation on CD4+ memory T lymphocytes during diapedesis, we used an in vitro model of the blood-brain barrier (BBB). We found that only memory (CD45RO+) cells transmigrated and type 3 CXC chemokine receptor (CXCR3) was enriched on transmigrated cells. CXCR3 depletion of the input population did not affect transmigration capability. CXCR3 reemerged on CXCR3 depleted cells independently of endothelial cell exposure, but was susceptible to incubation at 4°C, indicating receptor recycling. We propose that CXCR3 serves as a surface marker for cells that have the capacity to cross the BBB, but does not play an essential role in extravasation.
KW - Blood-brain barrier
KW - CXCR3
KW - Multiple sclerosis
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U2 - 10.1016/j.jneuroim.2004.05.004
DO - 10.1016/j.jneuroim.2004.05.004
M3 - Article
C2 - 15265673
AN - SCOPUS:3242726945
SN - 0165-5728
VL - 153
SP - 150
EP - 157
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
IS - 1-2
ER -