CXCR3 marks CD4+ memory T lymphocytes that are competent to migrate across a human brain microvascular endothelial cell layer

Melissa K. Callahan, Katherine A. Williams, Pia Kivisäkk, Donna Pearce, Monique F. Stins, Richard M. Ransohoff

Research output: Contribution to journalArticle

Abstract

Chemokines and their receptors may be implicated in leukocyte ingress into brain during inflammation observed during the course of multiple sclerosis (MS). To address receptor modulation on CD4+ memory T lymphocytes during diapedesis, we used an in vitro model of the blood-brain barrier (BBB). We found that only memory (CD45RO+) cells transmigrated and type 3 CXC chemokine receptor (CXCR3) was enriched on transmigrated cells. CXCR3 depletion of the input population did not affect transmigration capability. CXCR3 reemerged on CXCR3 depleted cells independently of endothelial cell exposure, but was susceptible to incubation at 4°C, indicating receptor recycling. We propose that CXCR3 serves as a surface marker for cells that have the capacity to cross the BBB, but does not play an essential role in extravasation.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalJournal of Neuroimmunology
Volume153
Issue number1-2
DOIs
StatePublished - Aug 1 2004

Keywords

  • Blood-brain barrier
  • CXCR3
  • Multiple sclerosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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