CXCL12 Induction of Inducible Nitric Oxide Synthase in Human CD8 T Cells

Jonathan C. Choy, Tai Yi, Deepak A. Rao, George Tellides, Karen Fox-Talbot, William M. Baldwin, Jordan S. Pober

Research output: Contribution to journalArticle

Abstract

Background: We reported previously that inducible nitric oxide synthase (iNOS) expression in graft-infiltrating human T cells that is confined to the bystander population contributes to T- cell-mediated rejection of allograft arteries in a humanized mouse model. Herein we examine whether CXCL12, a chemokine thought to contribute to recruitment of bystander T cells, induces iNOS in human CD8 T cells. Methods: Human CD8 T cells were treated with CXCL12 and iNOS expression was examined. Also, human allograft arteries were immunohistochemically stained for iNOS and CD8, and adjacent sections stained for CXCL12 to determine their localization in human tissues. Results: Resting human CD8 and CD4 T cells expressed the CXCR4, but not the CXCR7, receptor for CXCL12. Treatment with CXCL12 induced expression of both iNOS mRNA and protein in primary human CD8 T cells in a dose-dependent manner, but had no effect on CD4 T cells. Induction of iNOS expression in CD8 T cells was mediated by increased gene transcription. T-cell-receptor (TCR)-activated CD8 T cells rapidly downregulated CXCR4, which coincided with diminished ability of CXCL12 to induce iNOS in activated T cells. iNOS expression in infiltrating human CD8 T cells was spatially associated with CXCL12 expression both in the humanized mouse model of allograft artery rejection and in clinical specimens of coronary arteries displaying allograft vasculopathy. Conclusions: CXCL12 induces iNOS expression in human CD8 T cells and this response may contribute to allograft rejection.

Original languageEnglish (US)
Pages (from-to)1333-1339
Number of pages7
JournalJournal of Heart and Lung Transplantation
Volume27
Issue number12
DOIs
StatePublished - Dec 2008

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Nitric Oxide Synthase Type II
T-Lymphocytes
Allografts
Arteries
Chemokine CXCL12
T-Cell Antigen Receptor
Coronary Vessels
Down-Regulation

ASJC Scopus subject areas

  • Transplantation
  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

Choy, J. C., Yi, T., Rao, D. A., Tellides, G., Fox-Talbot, K., Baldwin, W. M., & Pober, J. S. (2008). CXCL12 Induction of Inducible Nitric Oxide Synthase in Human CD8 T Cells. Journal of Heart and Lung Transplantation, 27(12), 1333-1339. https://doi.org/10.1016/j.healun.2008.08.014

CXCL12 Induction of Inducible Nitric Oxide Synthase in Human CD8 T Cells. / Choy, Jonathan C.; Yi, Tai; Rao, Deepak A.; Tellides, George; Fox-Talbot, Karen; Baldwin, William M.; Pober, Jordan S.

In: Journal of Heart and Lung Transplantation, Vol. 27, No. 12, 12.2008, p. 1333-1339.

Research output: Contribution to journalArticle

Choy, JC, Yi, T, Rao, DA, Tellides, G, Fox-Talbot, K, Baldwin, WM & Pober, JS 2008, 'CXCL12 Induction of Inducible Nitric Oxide Synthase in Human CD8 T Cells', Journal of Heart and Lung Transplantation, vol. 27, no. 12, pp. 1333-1339. https://doi.org/10.1016/j.healun.2008.08.014
Choy, Jonathan C. ; Yi, Tai ; Rao, Deepak A. ; Tellides, George ; Fox-Talbot, Karen ; Baldwin, William M. ; Pober, Jordan S. / CXCL12 Induction of Inducible Nitric Oxide Synthase in Human CD8 T Cells. In: Journal of Heart and Lung Transplantation. 2008 ; Vol. 27, No. 12. pp. 1333-1339.
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AU - Baldwin, William M.

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N2 - Background: We reported previously that inducible nitric oxide synthase (iNOS) expression in graft-infiltrating human T cells that is confined to the bystander population contributes to T- cell-mediated rejection of allograft arteries in a humanized mouse model. Herein we examine whether CXCL12, a chemokine thought to contribute to recruitment of bystander T cells, induces iNOS in human CD8 T cells. Methods: Human CD8 T cells were treated with CXCL12 and iNOS expression was examined. Also, human allograft arteries were immunohistochemically stained for iNOS and CD8, and adjacent sections stained for CXCL12 to determine their localization in human tissues. Results: Resting human CD8 and CD4 T cells expressed the CXCR4, but not the CXCR7, receptor for CXCL12. Treatment with CXCL12 induced expression of both iNOS mRNA and protein in primary human CD8 T cells in a dose-dependent manner, but had no effect on CD4 T cells. Induction of iNOS expression in CD8 T cells was mediated by increased gene transcription. T-cell-receptor (TCR)-activated CD8 T cells rapidly downregulated CXCR4, which coincided with diminished ability of CXCL12 to induce iNOS in activated T cells. iNOS expression in infiltrating human CD8 T cells was spatially associated with CXCL12 expression both in the humanized mouse model of allograft artery rejection and in clinical specimens of coronary arteries displaying allograft vasculopathy. Conclusions: CXCL12 induces iNOS expression in human CD8 T cells and this response may contribute to allograft rejection.

AB - Background: We reported previously that inducible nitric oxide synthase (iNOS) expression in graft-infiltrating human T cells that is confined to the bystander population contributes to T- cell-mediated rejection of allograft arteries in a humanized mouse model. Herein we examine whether CXCL12, a chemokine thought to contribute to recruitment of bystander T cells, induces iNOS in human CD8 T cells. Methods: Human CD8 T cells were treated with CXCL12 and iNOS expression was examined. Also, human allograft arteries were immunohistochemically stained for iNOS and CD8, and adjacent sections stained for CXCL12 to determine their localization in human tissues. Results: Resting human CD8 and CD4 T cells expressed the CXCR4, but not the CXCR7, receptor for CXCL12. Treatment with CXCL12 induced expression of both iNOS mRNA and protein in primary human CD8 T cells in a dose-dependent manner, but had no effect on CD4 T cells. Induction of iNOS expression in CD8 T cells was mediated by increased gene transcription. T-cell-receptor (TCR)-activated CD8 T cells rapidly downregulated CXCR4, which coincided with diminished ability of CXCL12 to induce iNOS in activated T cells. iNOS expression in infiltrating human CD8 T cells was spatially associated with CXCL12 expression both in the humanized mouse model of allograft artery rejection and in clinical specimens of coronary arteries displaying allograft vasculopathy. Conclusions: CXCL12 induces iNOS expression in human CD8 T cells and this response may contribute to allograft rejection.

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