Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae

J. Allen Baron, Janice S. Chen, Valeria L Culotta

Research output: Contribution to journalArticle

Abstract

In eukaryotes, the Cu/Zn containing superoxide dismutase (SOD1) plays a critical role in oxidative stress protection as well as in signaling. We recently demonstrated a function for Saccharomyces cerevisiae Sod1p in signaling through CK1γ casein kinases and identified the essential proton ATPase Pma1p as one likely target. The connection between Sod1p and Pma1p was explored further by testing the impact of sod1Δ mutations on cells expressing mutant alleles of Pma1p that alter activity and/or post-translational regulation of this ATPase. We report here that sod1Δ mutations are lethal when combined with the T912D allele of Pma1p in the C-terminal regulatory domain. This "synthetic lethality" was reversed by intragenic suppressor mutations in Pma1p, including an A906G substitution that lies within the C-terminal regulatory domain and hyper-activates Pma1p. Surprisingly the effect of sod1Δ mutations on Pma1-T912D is not mediated through the Sod1p signaling pathway involving the CK1γ casein kinases. Rather, Sod1p sustains life of cells expressing Pma1-T912D through oxidative stress protection. The synthetic lethality of sod1Δ Pma1-T912D cells is suppressed by growing cells under low oxygen conditions or by treatments with manganese-based antioxidants. We now propose a model in which Sod1p maximizes Pma1p activity in two ways: one involving signaling through CK1γ casein kinases and an independent role for Sod1p in oxidative stress protection.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume462
Issue number3
DOIs
StatePublished - Jun 3 2015

Fingerprint

Casein Kinases
Yeast
Oxidative stress
Saccharomyces cerevisiae
Adenosine Triphosphatases
Protons
Oxidative Stress
Mutation
Alleles
Cells
Genetic Suppression
Manganese
Eukaryota
Superoxide Dismutase
Substitution reactions
Antioxidants
Oxygen
Superoxide Dismutase-1
Testing
Synthetic Lethal Mutations

Keywords

  • Casein kinase
  • Oxidative stress
  • PMA1
  • Reactive oxygen species
  • SOD1
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae. / Baron, J. Allen; Chen, Janice S.; Culotta, Valeria L.

In: Biochemical and Biophysical Research Communications, Vol. 462, No. 3, 03.06.2015, p. 251-256.

Research output: Contribution to journalArticle

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