Cutting edge: SWI/SNF mediates antisense Igh transcription and locus-wide accessibility in B cell precursors

Oleg A. Osipovich, Ramesh Subrahmanyam, Steven Pierce, Ranjan Sen, Eugene M. Oltz

Research output: Contribution to journalArticlepeer-review

Abstract

The stepwise process of Ag receptor gene assembly, termed V(D)J recombination, is coordinated during lymphocyte development by sweeping changes in chromatin that permit or deny access to a single recombinase enzyme. We now show that switching/sucrose nonfermenting (SWI/SNF) chromatin remodeling complexes are recruited to the Igh locus by an enhancer-dependent process and that these complexes are essential for generating recombinase accessibility throughout the locus. Depletion of SWI/SNF in pro-B cells also inhibits antisense transcription through all clusters of Igh gene segments, a pioneering process that has been implicated in the initial opening of chromatin. We conclude that SWI/SNF complexes play multiple roles in Igh gene assembly, ranging from initial locus activation to the spreading and maintenance of chromatin accessibility over large VH, DH, and J H domains.

Original languageEnglish (US)
Pages (from-to)1509-1513
Number of pages5
JournalJournal of Immunology
Volume183
Issue number3
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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