Abstract
The lack of persistence of transferred autologous mature lymphocytes in humans has been a major limitation to the application of effective cell transfer therapies. The results of a pilot clinical trial in 13 patients with metastatic melanoma suggested that conditioning with nonmyeloablative chemotherapy before adoptive transfer of activated tumor-reactive T cells enhances tumor regression and increases the overall rates of objective clinical responses. The present report examines the relationship between T cell persistence and tumor regression through analysis of the TCR β-chain V region gene products expressed in samples obtained from 25 patients treated with this protocol Sequence analysis demonstrated that there was a significant correlation between tumor regression and the degree of persistence in peripheral blood of adoptively transferred T cell clones, suggesting that inadequate T cell persistence may represent a major factor limiting responses to adoptive immunotherapy.
Original language | English (US) |
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Pages (from-to) | 7125-7130 |
Number of pages | 6 |
Journal | Journal of Immunology |
Volume | 173 |
Issue number | 12 |
DOIs | |
State | Published - Dec 15 2004 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology