Cutting edge: Association with IκB Kinase β regulates the subcellular localization of Homer3

Gayatri Yatherajam, Pinaki P. Banerjee, Kelly A. McCorkell, Laura A. Solt, Eric P. Hanson, Lisa A. Madge, Shin Kang, Paul F. Worley, Jordan S. Orange, Michael J. May

Research output: Contribution to journalArticle

Abstract

The signaling and adaptor protein Homer3 plays a role in controlling immune homeostasis and self-reactivity. Homer3 is recruited to the immune synapse (IS) following TCR ligation, although the mechanisms regulating this subcellular localization are unknown.We show that Homer3 specifically associates with a novel ubiquitin-like domain in the IκB kinase (IKK) β subunit of the IKK complex. Homer3 associates with IKKb in T cells and colocalizes with the IKK complex at the IS. However, Homer3 is not required for IKK activation, as NF-κB signaling is intact in Homer3-deficient T cells. Instead, the IKK complex recruits Homer3 to the IS following TCR engagement, and we present evidence that this association regulates actin dynamics in T cells. These findings identify a novel interaction between two major signaling proteins and reveal an unexpected NF-κB - independent function for the IKK complex in regulating the subcellular localization of Homer3.

Original languageEnglish (US)
Pages (from-to)2665-2669
Number of pages5
JournalJournal of Immunology
Volume185
Issue number5
DOIs
StatePublished - Sep 1 2010

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yatherajam, G., Banerjee, P. P., McCorkell, K. A., Solt, L. A., Hanson, E. P., Madge, L. A., Kang, S., Worley, P. F., Orange, J. S., & May, M. J. (2010). Cutting edge: Association with IκB Kinase β regulates the subcellular localization of Homer3. Journal of Immunology, 185(5), 2665-2669. https://doi.org/10.4049/jimmunol.0903488