Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics

Hisashi Narimatsu, Hiroyuki Kaji, Sergey Y. Vakhrushev, Henrik Clausen, Hui Zhang, Erika Noro, Akira Togayachi, Chiaki Nagai-Okatani, Atsushi Kuno, Xia Zou, Li Cheng, Sheng Ce Tao, Yangyang Sun

Research output: Contribution to journalArticle

Abstract

Glycoproteomics is an important recent advance in the field of glycoscience. In glycomics, glycan structures are comprehensively analyzed after glycans are released from glycoproteins. However, a major limitation of glycomics is the lack of insight into glycoprotein functions. The Biology/Disease-driven Human Proteome Project has a particular focus on biological and medical applications. Glycoproteomics technologies aimed at obtaining a comprehensive understanding of intact glycoproteins, i.e., the kind of glycan structures that are attached to particular amino acids and proteins, have been developed. This Review focuses on the recent progress of the technologies and their applications. First, the methods for large-scale identification of both N- and O-glycosylated proteins are summarized. Next, the progress of analytical methods for intact glycopeptides is outlined. MS/MS-based methods were developed for improving the sensitivity and speed of the mass spectrometer, in parallel with the software for complex spectrum assignment. In addition, a unique approach to identify intact glycopeptides using MS1-based accurate masses is introduced. Finally, as an advance of glycomics, two approaches to provide the spatial distribution of glycans in cells are described, i.e., MS imaging and lectin microarray. These methods allow rapid glycomic profiling of different types of biological samples and thus facilitate glycoproteomics.

Original languageEnglish (US)
JournalJournal of Proteome Research
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Glycomics
Polysaccharides
Technology
Glycoproteins
Glycopeptides
Medical applications
Mass spectrometers
Proteome
Microarrays
Lectins
Spatial distribution
Proteins
Software
Imaging techniques
Amino Acids

Keywords

  • glycan spatial distribution
  • glycome mapping
  • glycoprotein
  • glycoproteome
  • glycosylation site-mapping
  • lectin microarray
  • mass spectrometry
  • protein glycosylation
  • site-specific glycan analysis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics. / Narimatsu, Hisashi; Kaji, Hiroyuki; Vakhrushev, Sergey Y.; Clausen, Henrik; Zhang, Hui; Noro, Erika; Togayachi, Akira; Nagai-Okatani, Chiaki; Kuno, Atsushi; Zou, Xia; Cheng, Li; Tao, Sheng Ce; Sun, Yangyang.

In: Journal of Proteome Research, 01.01.2018.

Research output: Contribution to journalArticle

Narimatsu, H, Kaji, H, Vakhrushev, SY, Clausen, H, Zhang, H, Noro, E, Togayachi, A, Nagai-Okatani, C, Kuno, A, Zou, X, Cheng, L, Tao, SC & Sun, Y 2018, 'Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics', Journal of Proteome Research. https://doi.org/10.1021/acs.jproteome.8b00515
Narimatsu, Hisashi ; Kaji, Hiroyuki ; Vakhrushev, Sergey Y. ; Clausen, Henrik ; Zhang, Hui ; Noro, Erika ; Togayachi, Akira ; Nagai-Okatani, Chiaki ; Kuno, Atsushi ; Zou, Xia ; Cheng, Li ; Tao, Sheng Ce ; Sun, Yangyang. / Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics. In: Journal of Proteome Research. 2018.
@article{45105eb2093749cbb3bb0ae37541b6ae,
title = "Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics",
abstract = "Glycoproteomics is an important recent advance in the field of glycoscience. In glycomics, glycan structures are comprehensively analyzed after glycans are released from glycoproteins. However, a major limitation of glycomics is the lack of insight into glycoprotein functions. The Biology/Disease-driven Human Proteome Project has a particular focus on biological and medical applications. Glycoproteomics technologies aimed at obtaining a comprehensive understanding of intact glycoproteins, i.e., the kind of glycan structures that are attached to particular amino acids and proteins, have been developed. This Review focuses on the recent progress of the technologies and their applications. First, the methods for large-scale identification of both N- and O-glycosylated proteins are summarized. Next, the progress of analytical methods for intact glycopeptides is outlined. MS/MS-based methods were developed for improving the sensitivity and speed of the mass spectrometer, in parallel with the software for complex spectrum assignment. In addition, a unique approach to identify intact glycopeptides using MS1-based accurate masses is introduced. Finally, as an advance of glycomics, two approaches to provide the spatial distribution of glycans in cells are described, i.e., MS imaging and lectin microarray. These methods allow rapid glycomic profiling of different types of biological samples and thus facilitate glycoproteomics.",
keywords = "glycan spatial distribution, glycome mapping, glycoprotein, glycoproteome, glycosylation site-mapping, lectin microarray, mass spectrometry, protein glycosylation, site-specific glycan analysis",
author = "Hisashi Narimatsu and Hiroyuki Kaji and Vakhrushev, {Sergey Y.} and Henrik Clausen and Hui Zhang and Erika Noro and Akira Togayachi and Chiaki Nagai-Okatani and Atsushi Kuno and Xia Zou and Li Cheng and Tao, {Sheng Ce} and Yangyang Sun",
year = "2018",
month = "1",
day = "1",
doi = "10.1021/acs.jproteome.8b00515",
language = "English (US)",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics

AU - Narimatsu, Hisashi

AU - Kaji, Hiroyuki

AU - Vakhrushev, Sergey Y.

AU - Clausen, Henrik

AU - Zhang, Hui

AU - Noro, Erika

AU - Togayachi, Akira

AU - Nagai-Okatani, Chiaki

AU - Kuno, Atsushi

AU - Zou, Xia

AU - Cheng, Li

AU - Tao, Sheng Ce

AU - Sun, Yangyang

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Glycoproteomics is an important recent advance in the field of glycoscience. In glycomics, glycan structures are comprehensively analyzed after glycans are released from glycoproteins. However, a major limitation of glycomics is the lack of insight into glycoprotein functions. The Biology/Disease-driven Human Proteome Project has a particular focus on biological and medical applications. Glycoproteomics technologies aimed at obtaining a comprehensive understanding of intact glycoproteins, i.e., the kind of glycan structures that are attached to particular amino acids and proteins, have been developed. This Review focuses on the recent progress of the technologies and their applications. First, the methods for large-scale identification of both N- and O-glycosylated proteins are summarized. Next, the progress of analytical methods for intact glycopeptides is outlined. MS/MS-based methods were developed for improving the sensitivity and speed of the mass spectrometer, in parallel with the software for complex spectrum assignment. In addition, a unique approach to identify intact glycopeptides using MS1-based accurate masses is introduced. Finally, as an advance of glycomics, two approaches to provide the spatial distribution of glycans in cells are described, i.e., MS imaging and lectin microarray. These methods allow rapid glycomic profiling of different types of biological samples and thus facilitate glycoproteomics.

AB - Glycoproteomics is an important recent advance in the field of glycoscience. In glycomics, glycan structures are comprehensively analyzed after glycans are released from glycoproteins. However, a major limitation of glycomics is the lack of insight into glycoprotein functions. The Biology/Disease-driven Human Proteome Project has a particular focus on biological and medical applications. Glycoproteomics technologies aimed at obtaining a comprehensive understanding of intact glycoproteins, i.e., the kind of glycan structures that are attached to particular amino acids and proteins, have been developed. This Review focuses on the recent progress of the technologies and their applications. First, the methods for large-scale identification of both N- and O-glycosylated proteins are summarized. Next, the progress of analytical methods for intact glycopeptides is outlined. MS/MS-based methods were developed for improving the sensitivity and speed of the mass spectrometer, in parallel with the software for complex spectrum assignment. In addition, a unique approach to identify intact glycopeptides using MS1-based accurate masses is introduced. Finally, as an advance of glycomics, two approaches to provide the spatial distribution of glycans in cells are described, i.e., MS imaging and lectin microarray. These methods allow rapid glycomic profiling of different types of biological samples and thus facilitate glycoproteomics.

KW - glycan spatial distribution

KW - glycome mapping

KW - glycoprotein

KW - glycoproteome

KW - glycosylation site-mapping

KW - lectin microarray

KW - mass spectrometry

KW - protein glycosylation

KW - site-specific glycan analysis

UR - http://www.scopus.com/inward/record.url?scp=85055633999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055633999&partnerID=8YFLogxK

U2 - 10.1021/acs.jproteome.8b00515

DO - 10.1021/acs.jproteome.8b00515

M3 - Article

C2 - 30359034

AN - SCOPUS:85055633999

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

ER -