Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics

Hisashi Narimatsu, Hiroyuki Kaji, Sergey Y. Vakhrushev, Henrik Clausen, Hui Zhang, Erika Noro, Akira Togayachi, Chiaki Nagai-Okatani, Atsushi Kuno, Xia Zou, Li Cheng, Sheng Ce Tao, Yangyang Sun

Research output: Contribution to journalArticle

Abstract

Glycoproteomics is an important recent advance in the field of glycoscience. In glycomics, glycan structures are comprehensively analyzed after glycans are released from glycoproteins. However, a major limitation of glycomics is the lack of insight into glycoprotein functions. The Biology/Disease-driven Human Proteome Project has a particular focus on biological and medical applications. Glycoproteomics technologies aimed at obtaining a comprehensive understanding of intact glycoproteins, i.e., the kind of glycan structures that are attached to particular amino acids and proteins, have been developed. This Review focuses on the recent progress of the technologies and their applications. First, the methods for large-scale identification of both N- and O-glycosylated proteins are summarized. Next, the progress of analytical methods for intact glycopeptides is outlined. MS/MS-based methods were developed for improving the sensitivity and speed of the mass spectrometer, in parallel with the software for complex spectrum assignment. In addition, a unique approach to identify intact glycopeptides using MS1-based accurate masses is introduced. Finally, as an advance of glycomics, two approaches to provide the spatial distribution of glycans in cells are described, i.e., MS imaging and lectin microarray. These methods allow rapid glycomic profiling of different types of biological samples and thus facilitate glycoproteomics.

Original languageEnglish (US)
JournalJournal of Proteome Research
DOIs
StateAccepted/In press - Jan 1 2018

Keywords

  • glycan spatial distribution
  • glycome mapping
  • glycoprotein
  • glycoproteome
  • glycosylation site-mapping
  • lectin microarray
  • mass spectrometry
  • protein glycosylation
  • site-specific glycan analysis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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