Current state of the hybridoma technology

L. Pirofski, Arturo Casadevall, L. Rodriguez, L. S. Zuckier, M. D. Scharff

Research output: Contribution to journalArticle

Abstract

The first description of the hybridoma technology in 1975 seemed to hold enormous promise for the treatment of a variety of human diseases. The ability to produce monoclonal antibodies led to the availability of large amounts of homogeneous and predictable preparations of antibody. The potential to renew indefinitely a particular antibody surmounted many of the technical and regulatory problems that made polyclonal antisera difficult to use as therapeutic agents in man. The hybridoma technology seemed even more valuable as it became clear that it could lead to the generation of pure, highly specific antibodies from impure, poorly characterized antigens. Monoclonal antibodies have been extremely useful in basic investigations and have facilitated the development of new diagnostic tests for serum and tissue components and infectious agents. However, novel approaches are needed in order to provide more useful, less immunogenic antibodies which could be used routinely for passive immunization in the treatment of infections or for tumor targeting.

Original languageEnglish (US)
JournalJournal of Clinical Immunology
Volume10
Issue number6 Supplement
DOIs
Publication statusPublished - Nov 1990
Externally publishedYes

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Keywords

  • Hybridomas
  • hyperchimeras
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Pirofski, L., Casadevall, A., Rodriguez, L., Zuckier, L. S., & Scharff, M. D. (1990). Current state of the hybridoma technology. Journal of Clinical Immunology, 10(6 Supplement). https://doi.org/10.1007/BF00918686