Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado

Douglas Norris, Barbara J B Johnson, Joseph Piesman, Gary O. Maupin, Jessica L. Clark, William C. Black IV

Research output: Contribution to journalArticle

Abstract

In Colorado, Borrelia burgdorferi sensu stricto, the etiologic agent of Lyme disease, is maintained in an enzootic cycle between Ixodes spinipalpis ticks and Neotoma mexicana rats (27). The frequencies of flagellin (fla), 66- kDa protein (p66), and outer surface protein A (ospA) alleles were examined in 71 B. burgdorferi isolates from samples from Colorado. Approximately two- thirds of these samples were isolates from I. spinipalpis ticks that had been cultured in BSK-H medium prior to DNA extraction. The remaining samples were from total DNA extracted directly from infected I. spinipalpis ticks. A portion of each gene was amplified by PCR and screened for genetic variability by single-strand conformation polymorphism (SSCP) analysis. We identified three alleles in the fla gene, seven in the p66 gene, and seven in the ospA gene. Sequencing verified that the amplified products originated from B. burgdorferi template DNA and indicated 100% sensitivity and specificity of the SSCP analysis. The frequencies of the p66 and ospA alleles were significantly different between cultured and uncultured spirochetes. The number of three-locus genotypes and the genetic diversity of alleles at all loci were consistently lower in cultured spirochetes, suggesting that culturing of B. burgdorferi in BSK-H medium may select for specific genotypes.

Original languageEnglish (US)
Pages (from-to)2359-2364
Number of pages6
JournalJournal of Clinical Microbiology
Volume35
Issue number9
StatePublished - Sep 1997
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Ticks
Alleles
Genotype
Flagellin
Spirochaetales
Genes
DNA
Sigmodontinae
Ixodes
Lyme Disease
Sensitivity and Specificity
Polymerase Chain Reaction
OspA protein
Proteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Norris, D., Johnson, B. J. B., Piesman, J., Maupin, G. O., Clark, J. L., & Black IV, W. C. (1997). Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado. Journal of Clinical Microbiology, 35(9), 2359-2364.

Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado. / Norris, Douglas; Johnson, Barbara J B; Piesman, Joseph; Maupin, Gary O.; Clark, Jessica L.; Black IV, William C.

In: Journal of Clinical Microbiology, Vol. 35, No. 9, 09.1997, p. 2359-2364.

Research output: Contribution to journalArticle

Norris, D, Johnson, BJB, Piesman, J, Maupin, GO, Clark, JL & Black IV, WC 1997, 'Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado', Journal of Clinical Microbiology, vol. 35, no. 9, pp. 2359-2364.
Norris, Douglas ; Johnson, Barbara J B ; Piesman, Joseph ; Maupin, Gary O. ; Clark, Jessica L. ; Black IV, William C. / Culturing selects for specific genotypes of Borrelia burgdorferi in an enzootic cycle in Colorado. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 9. pp. 2359-2364.
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