Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization: Relationship to expression of Syk and SHIP-2

Sarbjit S. Saini, Miya Paterniti, Kavitha Vasagar, Scott P. Gibbons, Patricia M. Sterba, Becky M. Vonakis

Research output: Contribution to journalArticle

Abstract

Recently, signaling changes in the Fce{open}RI pathway involving inositol lipid phosphatases have been identified in the basophils of chronic idiopathic urticaria (CIU) subjects. Based on the profile of basophil Fce{open}RI-mediated histamine degranulation, we have segregated CIU subjects into two groups, CIU Responder (CIU R) or CIU Nonresponder (CIU NR). In the present study, we compared expression of SHIP-1, SHIP-2, and Syk protein to histamine release (HR) from mast cells (MC) cultured from the peripheral blood of CIU R, CIU NR, and normal subjects. The MC of CIU R donors contained significantly increased Syk and decreased SHIP-2 as compared to CIU NR (Syk: p = 0.038, SHIP-2: p = 0.038) and normals (Syk: p = 0.042, SHIP-2: p = 0.027). Spontaneous HR from CIU donors was increased two-fold compared to normals (p = 0.04). In summary, our results suggest a possible predilection for urticarial MC to spontaneously degranulate upon IgE sensitization contributing to the increased pruritis associated with CIU.

Original languageEnglish (US)
Pages (from-to)342-348
Number of pages7
JournalClinical Immunology
Volume132
Issue number3
DOIs
StatePublished - Sep 1 2009

Keywords

  • CD34 stem cell
  • FcεRI
  • Mast cell
  • Signal transduction
  • Urticaria

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Cultured peripheral blood mast cells from chronic idiopathic urticaria patients spontaneously degranulate upon IgE sensitization: Relationship to expression of Syk and SHIP-2'. Together they form a unique fingerprint.

  • Cite this