Cultured hippocampal pyramidal neurons express two kinds of GABA A receptors

Patrick S. Mangan, Chengsan Sun, Mackenzie Carpenter, Howard P. Goodkin, Werner Sieghart, Jaideep Kapur

Research output: Contribution to journalArticlepeer-review

Abstract

We combined a study of the subcellular distribution of the α1, α2, α4, β1, β2/3, γ2, and δ subunits of the GABAA receptor with an electrophysiological analysis of GABA A receptor currents to determine the types of receptors expressed on cultured hippocampal pyramidal neurons. The immunocytochemistry study demonstrated that α1, α2, β2/3, and γ2 subunits formed distinct clusters of various sizes, which were colocalized with clusters of glutamate decarboxylase (GAD) immunoreactivity at rates ranging from 22 to 58%. In contrast, α4, β1, and δ subunits were distributed diffusely over the cell soma and neuronal processes of cultured neurons and did not colocalize with the synaptic marker GAD. Whole-cell GABAA receptor currents were moderately sensitive to GABA and were modulated by diazepam. The whole-cell currents were also enhanced by the neurosteroid allopregnanolone (10 nM). Tonic currents, measured as changes in baseline current and noise, were sensitive to Zn2+, furosemide, and loreclezole; they were insensitive to diazepam. These studies suggest that two kinds of GABAA receptors are expressed on cultured hippocampal neurons. One kind of receptor formed clusters, which were present at GABAergic synapses and in the extrasynaptic membrane. The α1, α2, β2/3, and γ2 subunits were contained in clustered receptors. The second kind was distributed diffusely in the extrasynaptic membrane. The α4, β1, and δ subunits were contained in these diffusely distributed receptors. The properties of tonic currents recorded from these neurons were similar to those from recombinant receptors containing α4, β1, and δ subunits.

Original languageEnglish (US)
Pages (from-to)775-788
Number of pages14
JournalMolecular Pharmacology
Volume67
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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