Culture of bovine pulmonary artery endothelial cells on Gelfoam blocks

M. Centra, R. E. Ratych, G. L. Cao, J. Li, E. Williams, R. M. Taylor, G. M. Rosen

Research output: Contribution to journalArticle

Abstract

Conventional methods of endothelial cell culture on monolayers and beads require enzymatic digestion, traumatic scraping, or centrifugation to transfer cells to other experimental systems. Gelfoam, a porous gelatin block, not only supports the growth of bovine pulmonary artery endothelial cells but also allows the rapid transfer of cell-laden blocks from one experimental system to another with minimal intervention. This property has been shown to be especially useful for the rapid fixation of endothelial cells for microscopy using standard histologic methods. Histology confirmed that the trabecular nature of the substrate allows endothelial cells to line the interstices of the sponge matrix and grow in a configuration that simulates the appearance of the endothelium in small vessels and capillaries. The inoculation of 1 x 105 endothelial cells on 7.5 mg Gelfoam (24 x 8 x 2 mm blocks) was enhanced by fibroblast growth factor and resulted in cell attachment by day 2 with a cell doubling time of 1.7 days. In addition, endothelial cells completely infiltrated 1, 5 and 7.5 mg Gelfoam blocks, as verified by histology. Assays to quantify cell number and protein were easily performed. To facilitate cell counting, the Gelfoam matrix was rapidly removed by the addition of 0.05 mg/ml collagenase, a concentration that interfered minimally with the assay for cellular protein concentration. The data demonstrate that Gelfoam is a suitable support growth matrix for the in vitro culture of bovine pulmonary artery endothelial cells.

Original languageEnglish (US)
Pages (from-to)3117-3121
Number of pages5
JournalFASEB Journal
Volume6
Issue number12
DOIs
StatePublished - 1992

Keywords

  • Gelfoam
  • endothelial cells

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Centra, M., Ratych, R. E., Cao, G. L., Li, J., Williams, E., Taylor, R. M., & Rosen, G. M. (1992). Culture of bovine pulmonary artery endothelial cells on Gelfoam blocks. FASEB Journal, 6(12), 3117-3121. https://doi.org/10.1096/fasebj.6.12.1521742