Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI)

Xiang Lan Yao, Jiong Liu, Eleanor Lee, Geoffrey S.F. Ling, Joseph T. McCabe

Research output: Contribution to journalArticlepeer-review

Abstract

Cullin-5 (Cul-5), a member of the cullin gene family of scaffold proteins of E3 ubiquitin-ligase complexes, has a role in proteolysis and cell cycle regulation. We recently demonstrated that cul-5 mRNA is ubiquitously expressed in the central nervous system. The present study used quantitative real time polymerase chain reaction and western blotting to measure changes in cul-5 mRNA and Cul-5 protein expression, respectively, in the injured CNS in response to traumatic brain injury (TBI). cul-5 mRNA levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1 and 7, but not on Day 3 following TBI. In the ipsilateral hippocampus, cul-5 mRNA was significantly reduced on Day 1 after TBI. Cul-5 protein levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1-7 post-TBI while levels were significantly lower in the ipsilateral hippocampus on Days 3-7 post-TBI. Since Cul-5 is ubiquitously expressed in eukaryotic cells and is linked to proteasome-mediated protein degradation, it may have a role in CNS cell fate determination under conditions of traumatic stress.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalNeuroscience Letters
Volume409
Issue number1
DOIs
StatePublished - Nov 27 2006

Keywords

  • Cullin-5
  • E3-ligase
  • Gene expression
  • Proteasome
  • Protein degradation
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

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