Csa-19, a radiation-responsive human gene, identified by an unbiased two-gel cDNA library screening method in human cancer cells

Elizabeth K. Balcer-Kubiczek, Stephen J. Meltzer, Lin Huang Han, Xiao Feng Zhang, Zhong Ming Shi, George H. Harrison, John M. Abraham

Research output: Contribution to journalArticle

Abstract

A novel polymerase chain reaction (PCR)-based method was used to identify candidate genes whose expression is altered in cancer cells by ionizing radiation. Transcriptional induction of randomly selected genes in control versus irradiated human HL60 cells was compared. Among several complementary DNA (cDNA) clones recovered by this approach, one cDNA clone (CL68-5) was downregulated in X-irradiated HL60 cells but unaffected by 12-O-tetradecanoyl phorbol-13-acetate, forskolin, or cyclosporin-A. DNA sequencing of the CL68-5 cDNA revealed 100% nucleotide sequence homology to the reported human Csa-19, gene. Northern blot analysis of RNA from control and irradiated cells revealed the expression of a single 0.7-kilobase (kb) messenger RNA (mRNA) transcript. This 0.7-kb Csa-19 mRNA transcript was also expressed in a variety of human adult and corresponding fetal normal tissues. Moreover, when the effect of X- or fission neutron-irradiation on Csa-19 mRNA was compared in cultured human cells differing in p53 gene status (p53-/- versus p53+/+), downregulation of Csa-19 by X-rays or fission neutrons was similar in p53-wild type and p53-null cell lines. Our results provide the first known example of a radiation-responsive gene in human cancer cells whose expression is not associated with p53, adenylate cyclase or protein kinase C.

Original languageEnglish (US)
Pages (from-to)3051-3057
Number of pages7
JournalOncogene
Volume14
Issue number25
DOIs
StatePublished - Aug 22 1997

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Keywords

  • Csa-19
  • DNA damage
  • Fission neutron
  • Gene transcription
  • Signal transduction
  • X-ray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Balcer-Kubiczek, E. K., Meltzer, S. J., Han, L. H., Zhang, X. F., Shi, Z. M., Harrison, G. H., & Abraham, J. M. (1997). Csa-19, a radiation-responsive human gene, identified by an unbiased two-gel cDNA library screening method in human cancer cells. Oncogene, 14(25), 3051-3057. https://doi.org/10.1038/sj.onc.1201151