TY - JOUR
T1 - Crystallization of an anti-2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl monoclonal antibody Fab fragment with and without bound hapten
AU - Leahy, Daniel J.
AU - Hynes, Thomas R.
AU - McConnell, Harden M.
AU - Fox, Robert O.
N1 - Funding Information:
This work was supported hy ONIt contract NOoOI4-X(i- K-0388 to H.M.M. and an American( lancer Society grant (NJ?-631) to R.O.F. and H.M.M. D.,J.L. is a student in the Medical Scientist, Training Program (MSTP GM073651 1) We grat,efully acknowledge the assistancr of Drs Paul Phizackerley and Ethan Merritt: thtx St.anfortl Synchrotron Radiation Laboratory staff for t.heir assistance with the diffraction experiments; Professors John Johnson and Michael Rossmann and Mr Greg Kamer of the Macromolecular Crystallography Supercomputer Facility at Purdue University for their assistance in processing the diffraction films. Early studies were carried out while T.R.H. and R.O.F. were members of t)he Department of Cell Biology, Stanford Universit#yM edical School, Stanford, CA 94305, V.S.A.
PY - 1988/10/5
Y1 - 1988/10/5
N2 - The Fab fragment of a monoclonal antibody, AN02, specific for a 2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl hapten was crystallized both with and without bound hapten. Both crystals formed in phosphate-buffered saline (150 mm-NaCl, 10 mm-Na2PO4, 0·02% (w/v) NaN3 (pH 7·4) at 4 °C and diffracted beyond 2·2 Å resolution (Å = 0·1 nm). Fab with bound hapten crystallizes in space group P6122 or P6522, with cell dimensions a = b = 73·23 A ̊, c = 373·8 A ̊, α = β = 90 ° and γ = 120 °. Unoccupied Fab also crystallizes in space group P6122 or P6522 with cell dimensions a = 73 A ̊, c = 377 A ̊, α = β = 90 ° and γ = 120 °.
AB - The Fab fragment of a monoclonal antibody, AN02, specific for a 2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl hapten was crystallized both with and without bound hapten. Both crystals formed in phosphate-buffered saline (150 mm-NaCl, 10 mm-Na2PO4, 0·02% (w/v) NaN3 (pH 7·4) at 4 °C and diffracted beyond 2·2 Å resolution (Å = 0·1 nm). Fab with bound hapten crystallizes in space group P6122 or P6522, with cell dimensions a = b = 73·23 A ̊, c = 373·8 A ̊, α = β = 90 ° and γ = 120 °. Unoccupied Fab also crystallizes in space group P6122 or P6522 with cell dimensions a = 73 A ̊, c = 377 A ̊, α = β = 90 ° and γ = 120 °.
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U2 - 10.1016/0022-2836(88)90215-X
DO - 10.1016/0022-2836(88)90215-X
M3 - Article
C2 - 3210234
AN - SCOPUS:0024279331
SN - 0022-2836
VL - 203
SP - 829
EP - 830
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 3
ER -