Crystallization of an anti-2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl monoclonal antibody Fab fragment with and without bound hapten

Daniel J. Leahy, Thomas R. Hynes, Harden M. McConnell, Robert O. Fox

Research output: Contribution to journalArticlepeer-review

Abstract

The Fab fragment of a monoclonal antibody, AN02, specific for a 2,2,6,6-tetramethyl-1-piperidinyloxy-dinitrophenyl hapten was crystallized both with and without bound hapten. Both crystals formed in phosphate-buffered saline (150 mm-NaCl, 10 mm-Na2PO4, 0·02% (w/v) NaN3 (pH 7·4) at 4 °C and diffracted beyond 2·2 Å resolution (Å = 0·1 nm). Fab with bound hapten crystallizes in space group P6122 or P6522, with cell dimensions a = b = 73·23 A ̊, c = 373·8 A ̊, α = β = 90 ° and γ = 120 °. Unoccupied Fab also crystallizes in space group P6122 or P6522 with cell dimensions a = 73 A ̊, c = 377 A ̊, α = β = 90 ° and γ = 120 °.

Original languageEnglish (US)
Pages (from-to)829-830
Number of pages2
JournalJournal of molecular biology
Volume203
Issue number3
DOIs
StatePublished - Oct 5 1988

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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