Crystallization and X-ray diffraction studies of a 434 Cro-DNA complex

Cynthia Wolberger, Stephen C. Harrison

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals have been obtained of the bacteriophage 434 Cro protein bound to a synthetic DNA operator. An analysis of the packing shows that the complexes stack end-to-end along crystallographic axis, forming long rods with non-crystallographic 113 screw symmetry. The average number of DNA base-pairs per turn is 10.27, which is somewhat more overwound as compared with the 434 repressor-DNA crystals of Anderson et al. Diffraction extends to 3 Å along the rod direction and to 5 Å in perpendicular directions.

Original languageEnglish (US)
Pages (from-to)951-954
Number of pages4
JournalJournal of molecular biology
Volume196
Issue number4
DOIs
StatePublished - Aug 20 1987
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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