Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex

Thomas Li; Cynthia Wolberger; Martha Stark; Alexander D. Johnson

doi:10.1002/prot.340210210

Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex

Thomas Li, Cynthia Wolberger, Martha Stark, Alexander D. Johnson

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P6₁ or P6₅ with a = b = 133 Å, c = 45.4 Å. Crystals that are flash‐frozen at −179°C diffract to 2.7 Å along the c‐axis and to 2.4 Å in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. © 1995 Wiley‐Liss, Inc.

Original language	English (US)
Pages (from-to)	161-164
Number of pages	4
Journal	Proteins: Structure, Function, and Bioinformatics
Volume	21
Issue number	2
DOIs	https://doi.org/10.1002/prot.340210210
State	Published - Feb 1995

Keywords

MATa1
MATα2
X‐ray diffraction
a1
cocrystals
homeodomain
protein–DNA interactions
yeast
α2

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/prot.340210210

Cite this

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title = "Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex",

abstract = "Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P61 or P65 with a = b = 133 {\AA}, c = 45.4 {\AA}. Crystals that are flash‐frozen at −179°C diffract to 2.7 {\AA} along the c‐axis and to 2.4 {\AA} in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. {\textcopyright} 1995 Wiley‐Liss, Inc.",

keywords = "MATa1, MATα2, X‐ray diffraction, a1, cocrystals, homeodomain, protein–DNA interactions, yeast, α2",

author = "Thomas Li and Cynthia Wolberger and Martha Stark and Johnson, {Alexander D.}",

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volume = "21",

pages = "161--164",

journal = "Proteins: Structure, Function, and Bioinformatics",

issn = "0887-3585",

publisher = "Wiley-Liss Inc.",

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T1 - Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex

AU - Li, Thomas

AU - Wolberger, Cynthia

AU - Stark, Martha

AU - Johnson, Alexander D.

PY - 1995/2

Y1 - 1995/2

N2 - Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P61 or P65 with a = b = 133 Å, c = 45.4 Å. Crystals that are flash‐frozen at −179°C diffract to 2.7 Å along the c‐axis and to 2.4 Å in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. © 1995 Wiley‐Liss, Inc.

AB - Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P61 or P65 with a = b = 133 Å, c = 45.4 Å. Crystals that are flash‐frozen at −179°C diffract to 2.7 Å along the c‐axis and to 2.4 Å in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. © 1995 Wiley‐Liss, Inc.

KW - MATa1

KW - MATα2

KW - X‐ray diffraction

KW - a1

KW - cocrystals

KW - homeodomain

KW - protein–DNA interactions

KW - yeast

KW - α2

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U2 - 10.1002/prot.340210210

DO - 10.1002/prot.340210210

M3 - Article

C2 - 7777491

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SN - 0887-3585

VL - 21

SP - 161

EP - 164

JO - Proteins: Structure, Function, and Bioinformatics

JF - Proteins: Structure, Function, and Bioinformatics

IS - 2

ER -

Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex

Abstract

Keywords

ASJC Scopus subject areas

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