Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex

Thomas Li, Cynthia Wolberger, Martha Stark, Alexander D. Johnson

Research output: Contribution to journalArticle

Abstract

Crystals have been obtained of a ternary complex containing the yeast a1/α2 homeodomain heterodimer bound to a 21‐base pair DNA site containing two 5′ overhanging bases at each end. The crystals are grown from cobaltic hexamine and form in space group P61 or P65 with a = b = 133 Å, c = 45.4 Å. Crystals that are flash‐frozen at −179°C diffract to 2.7 Å along the c‐axis and to 2.4 Å in perpendicular directions. The crystals contain one protein–DNA complex in the crystallographic asymmetric unit. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)161-164
Number of pages4
JournalProteins: Structure, Function, and Bioinformatics
Volume21
Issue number2
DOIs
StatePublished - Feb 1995

Keywords

  • MATa1
  • MATα2
  • X‐ray diffraction
  • a1
  • cocrystals
  • homeodomain
  • protein–DNA interactions
  • yeast
  • α2

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Crystallization and preliminary X‐ray diffraction studies of an a1/α2/DNA ternary complex'. Together they form a unique fingerprint.

  • Cite this