TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction studies of an anti-4-hydroxy-3-nitrophenylacetic acid monoclonal antibody Fab fragment complexed with immunizing and heteroclitic haptens
AU - Yuhasz, Stacieann C.
AU - Ysern, Xavier
AU - Strand, Mette
AU - Amzel, L. Mario
PY - 1989/9/20
Y1 - 1989/9/20
N2 - The Fab fragment of the anti-4-hydroxy-3-nitrophenylacetic acid monoclonal antibody, 88C6/12, has been crystallized in the presence of the eliciting hapten, 4-hydroxy-3-nitrophenacetyl-ε-aminocaproic acid (NP-aminocap) and the heteroclitic iodinated analog, 4-hydroxy-3-iodo-5-nitrophenylacetyl-ε-aminocaproic acid (NIP-aminocap). Crystals obtained by precipitation with 32% ( w v) polyethylene glycol 3400 in the presence of 40 to 400 μm of either NP-aminocap or NIP-aminocap, belong to the orthorhombic space group P212121 with cell dimensions a = 81.2 A ̊, b = 86.9 A ̊, c = 131.1 A ̊. The cell volume suggests the presence of two molecules of the complex per asymmetric unit. Analysis of the Patterson function indicates that these two molecules are related by a local 2-fold axis parallel to the crystallographic b axis located at x = 0.218 and z = 0.25.
AB - The Fab fragment of the anti-4-hydroxy-3-nitrophenylacetic acid monoclonal antibody, 88C6/12, has been crystallized in the presence of the eliciting hapten, 4-hydroxy-3-nitrophenacetyl-ε-aminocaproic acid (NP-aminocap) and the heteroclitic iodinated analog, 4-hydroxy-3-iodo-5-nitrophenylacetyl-ε-aminocaproic acid (NIP-aminocap). Crystals obtained by precipitation with 32% ( w v) polyethylene glycol 3400 in the presence of 40 to 400 μm of either NP-aminocap or NIP-aminocap, belong to the orthorhombic space group P212121 with cell dimensions a = 81.2 A ̊, b = 86.9 A ̊, c = 131.1 A ̊. The cell volume suggests the presence of two molecules of the complex per asymmetric unit. Analysis of the Patterson function indicates that these two molecules are related by a local 2-fold axis parallel to the crystallographic b axis located at x = 0.218 and z = 0.25.
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U2 - 10.1016/0022-2836(89)90280-5
DO - 10.1016/0022-2836(89)90280-5
M3 - Article
C2 - 2585486
AN - SCOPUS:0024975092
SN - 0022-2836
VL - 209
SP - 319
EP - 321
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 2
ER -