Crystallization and preliminary X-ray diffraction studies of an anti-4-hydroxy-3-nitrophenylacetic acid monoclonal antibody Fab fragment complexed with immunizing and heteroclitic haptens

Stacieann C. Yuhasz, Xavier Ysern, Mette Strand, L. Mario Amzel

Research output: Contribution to journalArticlepeer-review

Abstract

The Fab fragment of the anti-4-hydroxy-3-nitrophenylacetic acid monoclonal antibody, 88C6/12, has been crystallized in the presence of the eliciting hapten, 4-hydroxy-3-nitrophenacetyl-ε-aminocaproic acid (NP-aminocap) and the heteroclitic iodinated analog, 4-hydroxy-3-iodo-5-nitrophenylacetyl-ε-aminocaproic acid (NIP-aminocap). Crystals obtained by precipitation with 32% ( w v) polyethylene glycol 3400 in the presence of 40 to 400 μm of either NP-aminocap or NIP-aminocap, belong to the orthorhombic space group P212121 with cell dimensions a = 81.2 A ̊, b = 86.9 A ̊, c = 131.1 A ̊. The cell volume suggests the presence of two molecules of the complex per asymmetric unit. Analysis of the Patterson function indicates that these two molecules are related by a local 2-fold axis parallel to the crystallographic b axis located at x = 0.218 and z = 0.25.

Original languageEnglish (US)
Pages (from-to)319-321
Number of pages3
JournalJournal of molecular biology
Volume209
Issue number2
DOIs
StatePublished - Sep 20 1989

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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