Crystallization and preliminary X-ray diffraction analysis of the Saccharomyces cerevisiae Ran-binding protein Mog1p

Rosanna P. Baker, Murray Stewart

Research output: Contribution to journalArticlepeer-review

Abstract

Mog1p binds the Ras-family GTPase Ran/Gsp1p, which has a central role in nucleocytoplasmic transport and cell-cycle progression. Overexpression of MOG1 is able to suppress temperature-sensitive gsp1 mutants in yeast; Δmog1 null mutants display temperature-sensitive defects in nuclear trafficking. Orthorhombic crystals of bacterially expressed Mog1p that diffract to beyond 2 Å resolution using synchrotron radiation have been obtained. The crystals have P212121 symmetry, with unit-cell parameters a = 39.67, b = 51.96, c = 112.23 Å, a Matthews coefficient of 2.44 Å3 Da-1, an estimated solvent content of 49.5% and one chain in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)229-231
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume56
Issue number2
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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