Crystallization and preliminary X-ray analysis of the O- carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

Inmaculada Gómez García, Caren L. Freel Meyers, Christopher T. Walsh, David M. Lawson

Research output: Contribution to journalArticle

Abstract

Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.

Original languageEnglish (US)
Pages (from-to)1000-1002
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number11
DOIs
StatePublished - Nov 18 2008

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Keywords

  • Antibiotic biosynthesis
  • NovN
  • Novobiocin
  • O-carbamoyltransferases
  • Streptomyces

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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