TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of the O- carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides
AU - Gómez García, Inmaculada
AU - Freel Meyers, Caren L.
AU - Walsh, Christopher T.
AU - Lawson, David M.
PY - 2008
Y1 - 2008
N2 - Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.
AB - Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.
KW - Antibiotic biosynthesis
KW - NovN
KW - Novobiocin
KW - O-carbamoyltransferases
KW - Streptomyces
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U2 - 10.1107/S1744309108030145
DO - 10.1107/S1744309108030145
M3 - Article
C2 - 18997325
AN - SCOPUS:55949111674
SN - 1744-3091
VL - 64
SP - 1000
EP - 1002
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -