Crystallization and preliminary X-ray analysis of the O- carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

Inmaculada Gómez García, Caren L Meyers, Christopher T. Walsh, David M. Lawson

Research output: Contribution to journalArticle

Abstract

Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.

Original languageEnglish (US)
Pages (from-to)1000-1002
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number11
DOIs
StatePublished - 2008

Fingerprint

Aminocoumarins
DNA Gyrase
Novobiocin
Bacterial DNA
Synchrotrons
X ray analysis
Streptomyces
Crystallization
X-Rays
crystallization
Anti-Bacterial Agents
Crystals
Enzymes
crystals
Proteins
x rays
biosynthesis
antibiotics
Biosynthesis
Crystal symmetry

Keywords

  • Antibiotic biosynthesis
  • NovN
  • Novobiocin
  • O-carbamoyltransferases
  • Streptomyces

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

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title = "Crystallization and preliminary X-ray analysis of the O- carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides",
abstract = "Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 {\AA} resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 {\AA} at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.",
keywords = "Antibiotic biosynthesis, NovN, Novobiocin, O-carbamoyltransferases, Streptomyces",
author = "{G{\'o}mez Garc{\'i}a}, Inmaculada and Meyers, {Caren L} and Walsh, {Christopher T.} and Lawson, {David M.}",
year = "2008",
doi = "10.1107/S1744309108030145",
language = "English (US)",
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pages = "1000--1002",
journal = "Acta Crystallographica Section F:Structural Biology Communications",
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publisher = "John Wiley and Sons Ltd",
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T1 - Crystallization and preliminary X-ray analysis of the O- carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

AU - Gómez García, Inmaculada

AU - Meyers, Caren L

AU - Walsh, Christopher T.

AU - Lawson, David M.

PY - 2008

Y1 - 2008

N2 - Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.

AB - Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 Å resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 Å at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.

KW - Antibiotic biosynthesis

KW - NovN

KW - Novobiocin

KW - O-carbamoyltransferases

KW - Streptomyces

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