Crystallization and preliminary X-ray analysis of soybean lipoxygenase-1, a non-heme iron-containing dioxygenase

Crystals of lipoxygenase-1 from soybeans have been grown by the method of vapor diffusion in the presence of sodium formate, ammonium acetate, and lithium chloride at pH 7.0. This enzyme contains a non-heme iron and is closely related to a human lipoxygenase found in leukocytes that participates in the biosynthesis of leukotrienes and lipoxins. The crystals are monoclinic space group C2 with cell dimensions of a = 183.8 Å, b = 123.2 Å c = 94.3 Å and β = 102.9 °. They diffract beyond 2.7 Å, are stable for several days in the x-ray beam, and appear to be suitable for x-ray diffraction studies.