Crystallization and preliminary X-ray analysis of soybean lipoxygenase-1, a non-heme iron-containing dioxygenase

J. C. Boyington, B. J. Gaffney, L. M. Amzel

Research output: Contribution to journalArticlepeer-review

Abstract

Crystals of lipoxygenase-1 from soybeans have been grown by the method of vapor diffusion in the presence of sodium formate, ammonium acetate, and lithium chloride at pH 7.0. This enzyme contains a non-heme iron and is closely related to a human lipoxygenase found in leukocytes that participates in the biosynthesis of leukotrienes and lipoxins. The crystals are monoclinic space group C2 with cell dimensions of a = 183.8 Å, b = 123.2 Å, c = 94.3 Å and β = 102.9°. They diffract beyond 2.7 Å, are stable for several days in the x-ray beam, and appear to be suitable for x-ray diffraction studies.

Original languageEnglish (US)
Pages (from-to)12771-12773
Number of pages3
JournalJournal of Biological Chemistry
Volume265
Issue number22
StatePublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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