Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, αLβ2) integrin

Aidong Qu, Daniel J. Leahy

Research output: Contribution to journalArticle

Abstract

We report the 1.8-Å crystal structure of the CD11a I-domain with bound manganese ion. The CD11a I-domain contains binding sites for intercellular adhesion molecules 1 and 3 and can exist in both low- and high-affinity states. The metal-bound form reported here is likely to represent a high-affinity state. The CD11a I-domain structure reveals a strained hydrophobic ridge adjacent to the bound metal ion that may serve as a ligand-binding surface and is likely to rearrange in the absence of bound metal ion. The CD11a I-domain is homologous to domains found in von Willebrand factor, and mapping of mutations found in types 2a and 2b von Willebrand disease onto this structure allows consideration of the molecular basis of these forms of the disease.

Original languageEnglish (US)
Pages (from-to)10277-10281
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number22
DOIs
StatePublished - Oct 24 1995

Keywords

  • Cell-cell adhesion
  • Metal-binding site
  • Protein structure
  • Von Willebrand disease

ASJC Scopus subject areas

  • General

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