@article{2786356ddaef4881ab626698f42ddd54,
title = "Crystal structure of a soluble form of the human T cell coreceptor CD8 at 2.6 {\AA} resolution",
abstract = "A secreted fragment of the extracellular portion of human CD8α has been expressed in CHO cells, and a deglycosylated and proteolyzed form of this fragment has been crystallized. We report here the crystal structure of this fragment as refined at 2.6 {\AA} resolution. The structure was solved by molecular replacement using a superposition of ten variable domains from immunoglobulin light chains as the search model. Only the N-terminal 114 amino acids of CD8α are visible in the electron density maps. The domain formed by these residues possesses a fold typical of immunoglobulin variable domains and associates to form Fv-like homodimers.",
author = "Leahy, {Daniel J.} and Richard Axel and Hendrickson, {Wayne A.}",
note = "Funding Information: We thank Dan Denney for the gift of a plasmid containing the cytomeg-altovirus intermediate/early promoter/enhancer and SV40 splice site sequences, Adelaide Caruthers for the DG44 cell line, Tom Livelli for advice and assistance with transfections, Lillian Eoyang and Leigh Zawel for technical assistance, Mary Ann Gawinowicz for N-terminal sequence analysis, Andrew Pound for amino acid analyses, Susan Pollak for carbohydrate analyses, Rachel Yarmolinsky for drawing the ribbon diagram of CD8 found in Figure 5, Kurt Drickamer and Ellen Robey for helpful discussions, and Tom Peat, Bill Weis. and Wei Yang for assistance with X-ray data collection. We gratefully acknowledge use of the CHESS facilihes. This work was supported in part by National Institutes of Health grant 5-31294-3600 to R. A. and by National Science Foundation grant DMB-89-17570 to W. A. H.; D. J. L. is a fellow of the Helen Hay Whitney Foundation. Atomic coordinates and structure factor amplitudes have been deposited with the Protein Data Bank, Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973, from which copies are available.",
year = "1992",
month = mar,
day = "20",
doi = "10.1016/0092-8674(92)90085-Q",
language = "English (US)",
volume = "68",
pages = "1145--1162",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}