TY - JOUR
T1 - Cryptosporidium Species and Genotypes in HIV-Positive Patients in Lima, Peru
AU - Cama, Vitaliano A.
AU - Bern, Caryn
AU - Sulaiman, I. M.
AU - Gilman, Robert H.
AU - Ticona, Eduardo
AU - Vivar, Aldo
AU - Kawai, Vivian
AU - Vargas, Daniel
AU - Zhou, Ling
AU - Xiao, Lihua
PY - 2003/11
Y1 - 2003/11
N2 - Cryptosporidium parasites from a cross-sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to determine the diversity of Cryptosporidium spp. in HIV-positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium-positive specimens from 300 patients using a small subunit rRNA-based PCR-RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV-positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.
AB - Cryptosporidium parasites from a cross-sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to determine the diversity of Cryptosporidium spp. in HIV-positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium-positive specimens from 300 patients using a small subunit rRNA-based PCR-RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV-positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.
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U2 - 10.1111/j.1550-7408.2003.tb00620.x
DO - 10.1111/j.1550-7408.2003.tb00620.x
M3 - Article
C2 - 14736153
AN - SCOPUS:9144265674
SN - 1066-5234
VL - 50
SP - 531
EP - 533
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
IS - SUPPL.
ER -