Cryptococcus neoformans var. gattii can exploit Acanthamoeba castellanii for growth

Stephanie D. Malliaris, Judith N. Steenbergen, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

It has recently been proposed that the origin and maintenance of virulence in certain environmental fungi is influenced by their interactions with non-vertebrate hosts such as amoebae and nematodes. In prior studies we have shown that the interactions of the soil amoebae Acanthamoeba castellanii with Cryptococcus neoformans varieties neoformans and grubii resemble those with macrophages. Here we extend those studies to C. neoformans variety gattii and describe quantitative differences in the type and outcome of the interactions observed relative to the other varieties. C. neoformans var. gattii proliferated in the presence of A. castellanii but the interaction was primarily extracellular with a paucity of phagocytic events. Experiments with acapsular cells coated with polysaccharide suggest that differences in the capsule structure may be responsible for the different interactions between cells of varieties neoformans, grubii, and gattii with amoebae. The ability of C. neoformans var. gattii to exploit amoebae indicates that despite major biological differences between C. neoformans varieties, all retain the ability to be pathogenic for A. castellanii.

Original languageEnglish (US)
Pages (from-to)149-158
Number of pages10
JournalMedical Mycology
Volume42
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Acanthamoeba castellanii
Cryptococcus neoformans
Amoeba
Growth
Cell Communication
Capsules
Polysaccharides
Virulence
macrophages
Fungi
virulence
Soil
polysaccharides
Macrophages
Maintenance
Nematoda
cells
fungi
soil

Keywords

  • C. neoformans var. gattii
  • Environmental
  • Phagocytosis
  • Virulence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • veterinary(all)
  • Microbiology

Cite this

Cryptococcus neoformans var. gattii can exploit Acanthamoeba castellanii for growth. / Malliaris, Stephanie D.; Steenbergen, Judith N.; Casadevall, Arturo.

In: Medical Mycology, Vol. 42, No. 2, 04.2004, p. 149-158.

Research output: Contribution to journalArticle

Malliaris, Stephanie D. ; Steenbergen, Judith N. ; Casadevall, Arturo. / Cryptococcus neoformans var. gattii can exploit Acanthamoeba castellanii for growth. In: Medical Mycology. 2004 ; Vol. 42, No. 2. pp. 149-158.
@article{5be4793f3fee4cbf9cd5757dd25e76be,
title = "Cryptococcus neoformans var. gattii can exploit Acanthamoeba castellanii for growth",
abstract = "It has recently been proposed that the origin and maintenance of virulence in certain environmental fungi is influenced by their interactions with non-vertebrate hosts such as amoebae and nematodes. In prior studies we have shown that the interactions of the soil amoebae Acanthamoeba castellanii with Cryptococcus neoformans varieties neoformans and grubii resemble those with macrophages. Here we extend those studies to C. neoformans variety gattii and describe quantitative differences in the type and outcome of the interactions observed relative to the other varieties. C. neoformans var. gattii proliferated in the presence of A. castellanii but the interaction was primarily extracellular with a paucity of phagocytic events. Experiments with acapsular cells coated with polysaccharide suggest that differences in the capsule structure may be responsible for the different interactions between cells of varieties neoformans, grubii, and gattii with amoebae. The ability of C. neoformans var. gattii to exploit amoebae indicates that despite major biological differences between C. neoformans varieties, all retain the ability to be pathogenic for A. castellanii.",
keywords = "C. neoformans var. gattii, Environmental, Phagocytosis, Virulence",
author = "Malliaris, {Stephanie D.} and Steenbergen, {Judith N.} and Arturo Casadevall",
year = "2004",
month = "4",
doi = "10.1080/13693786310001616500",
language = "English (US)",
volume = "42",
pages = "149--158",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Cryptococcus neoformans var. gattii can exploit Acanthamoeba castellanii for growth

AU - Malliaris, Stephanie D.

AU - Steenbergen, Judith N.

AU - Casadevall, Arturo

PY - 2004/4

Y1 - 2004/4

N2 - It has recently been proposed that the origin and maintenance of virulence in certain environmental fungi is influenced by their interactions with non-vertebrate hosts such as amoebae and nematodes. In prior studies we have shown that the interactions of the soil amoebae Acanthamoeba castellanii with Cryptococcus neoformans varieties neoformans and grubii resemble those with macrophages. Here we extend those studies to C. neoformans variety gattii and describe quantitative differences in the type and outcome of the interactions observed relative to the other varieties. C. neoformans var. gattii proliferated in the presence of A. castellanii but the interaction was primarily extracellular with a paucity of phagocytic events. Experiments with acapsular cells coated with polysaccharide suggest that differences in the capsule structure may be responsible for the different interactions between cells of varieties neoformans, grubii, and gattii with amoebae. The ability of C. neoformans var. gattii to exploit amoebae indicates that despite major biological differences between C. neoformans varieties, all retain the ability to be pathogenic for A. castellanii.

AB - It has recently been proposed that the origin and maintenance of virulence in certain environmental fungi is influenced by their interactions with non-vertebrate hosts such as amoebae and nematodes. In prior studies we have shown that the interactions of the soil amoebae Acanthamoeba castellanii with Cryptococcus neoformans varieties neoformans and grubii resemble those with macrophages. Here we extend those studies to C. neoformans variety gattii and describe quantitative differences in the type and outcome of the interactions observed relative to the other varieties. C. neoformans var. gattii proliferated in the presence of A. castellanii but the interaction was primarily extracellular with a paucity of phagocytic events. Experiments with acapsular cells coated with polysaccharide suggest that differences in the capsule structure may be responsible for the different interactions between cells of varieties neoformans, grubii, and gattii with amoebae. The ability of C. neoformans var. gattii to exploit amoebae indicates that despite major biological differences between C. neoformans varieties, all retain the ability to be pathogenic for A. castellanii.

KW - C. neoformans var. gattii

KW - Environmental

KW - Phagocytosis

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=1842863497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842863497&partnerID=8YFLogxK

U2 - 10.1080/13693786310001616500

DO - 10.1080/13693786310001616500

M3 - Article

C2 - 15124868

AN - SCOPUS:1842863497

VL - 42

SP - 149

EP - 158

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 2

ER -