Cryptococcus neoformans capsular enlargement and cellular gigantism during Galleria mellonella infection

Rocío García-Rodas, Arturo Casadevall, Juan Luís Rodríguez-Tudela, Manuel Cuenca-Estrella, Oscar Zaragoza

Research output: Contribution to journalArticle

Abstract

We have studied infection of Cryptococcus neoformans in the non-vertebrate host Galleria mellonella with particular interest in the morphological response of the yeast. Inoculation of C. neoformans in caterpillars induced a capsule-independent increase in haemocyte density 2 h after infection. C. neoformans manifested a significant increase in capsule size after inoculation into the caterpillar. The magnitude of capsule increase depended on the temperature, being more pronounced at 37°C than at 30°C, which correlated with an increased virulence of the fungus and reduced phagocytosis at 37°C. Capsule enlargement impaired phagocytosis by haemocytes. Incubation of the yeast in G. mellonella extracts also resulted in capsule enlargement, with the polar lipidic fraction having a prominent role in this effect. During infection, the capsule decreased in permeability. A low proportion of the cells (

Original languageEnglish (US)
Article numbere24485
JournalPLoS One
Volume6
Issue number9
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Gigantism
Cryptococcus neoformans
Galleria mellonella
Capsules
hemocytes
phagocytosis
insect larvae
Infection
infection
yeasts
Hemocytes
Phagocytosis
Yeast
permeability
virulence
Yeasts
fungi
extracts
Fungi
Virulence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cryptococcus neoformans capsular enlargement and cellular gigantism during Galleria mellonella infection. / García-Rodas, Rocío; Casadevall, Arturo; Rodríguez-Tudela, Juan Luís; Cuenca-Estrella, Manuel; Zaragoza, Oscar.

In: PLoS One, Vol. 6, No. 9, e24485, 2011.

Research output: Contribution to journalArticle

García-Rodas, Rocío ; Casadevall, Arturo ; Rodríguez-Tudela, Juan Luís ; Cuenca-Estrella, Manuel ; Zaragoza, Oscar. / Cryptococcus neoformans capsular enlargement and cellular gigantism during Galleria mellonella infection. In: PLoS One. 2011 ; Vol. 6, No. 9.
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