Cryopreservation of human prophase I oocytes collected from unstimulated follicles

T. L. Toth, S. E. Lanzendorf, B. A. Sandow, L. L. Veeck, W. A. Hassen, K. Hansen, G. D. Hodgen

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the cryopreservation of immature human oocytes obtained from unstimulated ovarian tissue. Design: Immature prophase I oocytes were obtained from unstimulated follicles and were either cryopreserved or cultured as controls. Cryopreservation was performed in a programmable freezing machine using one of two protocols. Method I (n = 133) used a one-step addition of cryoprotectant followed by a slow freeze and thaw protocol. With method II (n = 95), the cryoprotectant was added in a stepwise manner with cryopreservation performed in the presence of 0.2 M sucrose followed by rapid freezing and thawing. Setting: Basic research center at a medical school. Patients: Patients undergoing oophorectomy for nonovarian pathology. Main Outcome Measures: Rates of survival and maturation to metaphase II were compared between control oocytes and oocytes cryopreserved with methods I and II. Results: With method I, a survival rate of 15.6% was obtained with 58.3% of surviving oocytes reaching metaphase II after culture compared with 50.0% of nonfrozen control oocytes. Method II produced a survival rate of 43.3% with 27.3% maturing to metaphase II. Maturation of control oocytes for method II was 46.4%. Although the survival rate with method II was significantly higher than with method I, the rate of in vitro maturation to metaphase II showed no difference. Conclusions: These results demonstrate that human prophase I oocytes obtained from unstimulated antral follicles are capable of meiotic maturation after cryopreservation.

Original languageEnglish (US)
Pages (from-to)1077-1082
Number of pages6
JournalFertility and sterility
Volume61
Issue number6
DOIs
StatePublished - Jan 1 1994

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Keywords

  • In vitro maturation
  • cryopreservation
  • oocyte
  • transmission electron microscopy

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Toth, T. L., Lanzendorf, S. E., Sandow, B. A., Veeck, L. L., Hassen, W. A., Hansen, K., & Hodgen, G. D. (1994). Cryopreservation of human prophase I oocytes collected from unstimulated follicles. Fertility and sterility, 61(6), 1077-1082. https://doi.org/10.1016/s0015-0282(16)56759-4