Cryo-electron tomography of bacteria: Progress, challenges and future prospects

Jacqueline L S Milne; Sriram Subramaniam

doi:10.1038/nrmicro2183

Cryo-electron tomography of bacteria: Progress, challenges and future prospects

Jacqueline L S Milne, Sriram Subramaniam

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

Recent advances in three-dimensional electron microscopy provide remarkable tools to image the interior of bacterial cells. Glimpses of cells at resolutions that are 1-2 orders of magnitude higher than those currently attained with light microscopy can now be obtained with cryo-electron tomography, especially when used in combination with new tools for image averaging. This Review highlights recent advances in this area and provides an assessment of the general applicability, current limitations and type of structural information that can be obtained about the organization of intact cells using tomography. Possible future directions for whole cell imaging are also discussed.

Original language	English (US)
Pages (from-to)	666-675
Number of pages	10
Journal	Nature Reviews Microbiology
Volume	7
Issue number	9
DOIs	https://doi.org/10.1038/nrmicro2183
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

General Immunology and Microbiology

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10.1038/nrmicro2183

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title = "Cryo-electron tomography of bacteria: Progress, challenges and future prospects",

abstract = "Recent advances in three-dimensional electron microscopy provide remarkable tools to image the interior of bacterial cells. Glimpses of cells at resolutions that are 1-2 orders of magnitude higher than those currently attained with light microscopy can now be obtained with cryo-electron tomography, especially when used in combination with new tools for image averaging. This Review highlights recent advances in this area and provides an assessment of the general applicability, current limitations and type of structural information that can be obtained about the organization of intact cells using tomography. Possible future directions for whole cell imaging are also discussed.",

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AB - Recent advances in three-dimensional electron microscopy provide remarkable tools to image the interior of bacterial cells. Glimpses of cells at resolutions that are 1-2 orders of magnitude higher than those currently attained with light microscopy can now be obtained with cryo-electron tomography, especially when used in combination with new tools for image averaging. This Review highlights recent advances in this area and provides an assessment of the general applicability, current limitations and type of structural information that can be obtained about the organization of intact cells using tomography. Possible future directions for whole cell imaging are also discussed.

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Cryo-electron tomography of bacteria: Progress, challenges and future prospects

Abstract

ASJC Scopus subject areas

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