Crustacean lipovitellin. Isolation and characterization of the major high-density lipoprotein from the eggs of decapods

Robin A. Wallace, Susan L. Walker, Peter V. Hauschka

Research output: Contribution to journalArticle

Abstract

A simple procedure is described by which the principal protein component of the eggs and mature ovaries of six decapod crustaceans was isolated in relatively pure form and in large amounts. The component, in all cases, was a lipoprotein (30% lipid) which lacked protein-bound phosphorus and had an average molecular weight of 3.5 × 105. The relatively intense chromatic properties of the crustacean lipoproteins were due to the presence of a carotenoid noncovalently bonded to the lipid and/or protein. Those proteins displaying colors (purple, blue, andgreen) associated with shorter wavelengths of the visible spectrum undergo spectral changes dependent upon solvent conditions. Although these crustacean proteins may not be homologous to the vertebrate lipovitellins, they appear to serve in the same capacity, and generic use of the term "lipovitellin" is thus suggested for the major high-density lipoprotein found within animal eggs. The crustacean lipovitellins appear to offer several advantages for studies of lipoproteins in general and lipoprotein synthesis in particular.

Original languageEnglish (US)
Pages (from-to)1582-1590
Number of pages9
JournalBiochemistry®
Volume6
Issue number6
StatePublished - 1967
Externally publishedYes

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Decapoda (Crustacea)
HDL Lipoproteins
Eggs
Lipoproteins
Proteins
Arthropod Proteins
Color
Lipids
Egg Proteins
Carotenoids
Phosphorus
Vertebrates
Ovary
Animals
Molecular Weight
Molecular weight
Wavelength
lipovitellin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Crustacean lipovitellin. Isolation and characterization of the major high-density lipoprotein from the eggs of decapods. / Wallace, Robin A.; Walker, Susan L.; Hauschka, Peter V.

In: Biochemistry®, Vol. 6, No. 6, 1967, p. 1582-1590.

Research output: Contribution to journalArticle

Wallace, Robin A. ; Walker, Susan L. ; Hauschka, Peter V. / Crustacean lipovitellin. Isolation and characterization of the major high-density lipoprotein from the eggs of decapods. In: Biochemistry®. 1967 ; Vol. 6, No. 6. pp. 1582-1590.
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