Crowders perturb the entropy of RNA energy landscapes to favor folding

Duncan Kilburn, Joon Ho Roh, Reza Behrouzi, Robert M. Briber, Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

Biological macromolecules have evolved to fold and operate in the crowded environment of the cell. We have shown previously that molecular crowding stabilizes folded RNA structures. Here we report SAXS measurements on a 64 kDa bacterial group I ribozyme in the presence of mono- and divalent ions and PEG crowders of different molecular weight. These experiments show that crowders always stabilize the folded RNA, but this stabilization is weaker in NaCl solutions than MgCl2 solutions. Additionally, we find that RNAs with the same global structure, parametrized by Rg, have different scattering functions depending upon the ratio of electrostatic and entropic stabilization by ions and crowders, respectively. We quantify this difference using the scattering length per scattering volume and find that this ratio is larger for RNAs that fold in lower ionic strength solutions due to the higher crowder content. We conclude that lower RNA flexibility, or reduced configurational entropy, widens the free energy gap between the unfolded and folded RNA in crowded MgCl2 solutions.

Original languageEnglish (US)
Pages (from-to)10055-10063
Number of pages9
JournalJournal of the American Chemical Society
Volume135
Issue number27
DOIs
StatePublished - Jul 10 2013

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Entropy
RNA
Magnesium Chloride
Scattering
RNA Folding
Ions
Stabilization
Static Electricity
Osmolar Concentration
Ionic strength
Macromolecules
Polyethylene glycols
Free energy
Molecular Weight
Electrostatics
Energy gap
Molecular weight
Experiments

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Kilburn, D., Roh, J. H., Behrouzi, R., Briber, R. M., & Woodson, S. A. (2013). Crowders perturb the entropy of RNA energy landscapes to favor folding. Journal of the American Chemical Society, 135(27), 10055-10063. https://doi.org/10.1021/ja4030098

Crowders perturb the entropy of RNA energy landscapes to favor folding. / Kilburn, Duncan; Roh, Joon Ho; Behrouzi, Reza; Briber, Robert M.; Woodson, Sarah A.

In: Journal of the American Chemical Society, Vol. 135, No. 27, 10.07.2013, p. 10055-10063.

Research output: Contribution to journalArticle

Kilburn, D, Roh, JH, Behrouzi, R, Briber, RM & Woodson, SA 2013, 'Crowders perturb the entropy of RNA energy landscapes to favor folding', Journal of the American Chemical Society, vol. 135, no. 27, pp. 10055-10063. https://doi.org/10.1021/ja4030098
Kilburn, Duncan ; Roh, Joon Ho ; Behrouzi, Reza ; Briber, Robert M. ; Woodson, Sarah A. / Crowders perturb the entropy of RNA energy landscapes to favor folding. In: Journal of the American Chemical Society. 2013 ; Vol. 135, No. 27. pp. 10055-10063.
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