Crosstalk between spinal astrocytes and nuerons in nerve injury-injuced neuropathic pain

Wei Wang, Wen Wang, Xiaopeng Mei, Jing Huang, Yanyan Wei, Yayun Wang, Shengxi Wu, Yunqing Li

Research output: Contribution to journalArticle

Abstract

Emerging research implicates the participation of spinal dorsal horn (SDH) neurons and astrocytes in nerve injury-induced neuropathic pain. However, the crosstalk between spinal astrocytes and neurons in neuropathic pain is not clear. Using a lumbar 5 (L5) spinal nerve ligation (SNL) pain model, we testified our hypothesis that SDH neurons and astrocytes reciprocally regulate each other to maintain the persistent neuropathic pain states. Glial fibrillary acidic protein (GFAP) was used as the astrocytic specific marker and Fos, protein of the protooncogene c-fos, was used as a marker for activated neurons. SNL induced a significant mechanical allodynia as well as activated SDH neurons indicated by the Fos expression at the early phase and activated astrocytes with the increased expression of GFAP during the late phase of pain, respectively. Intrathecal administration of c-fos antisense oligodeoxynucleotides (ASO) or astroglial toxin L-α-aminoadipate (L-AA) reversed the mechanical allodynia, respectively. Immunofluorescent histochemistry revealed that intrathecal administration of c-fos ASO significantly suppressed activation of not only neurons but also astrocytes induced by SNL. Meanwhile, L-AA shortened the duration of neuronal activation by SNL. Our data offers evidence that neuronal and astrocytic activations are closely related with the maintenance of neuropathic pain through a reciprocal "crosstalk". The current study suggests that neuronal and non-neuronal elements should be taken integrally into consideration for nociceptive transmission, and that the intervention of such interaction may offer some novel pain therapeutic strategies.

Original languageEnglish (US)
Article numbere6973
JournalPLoS One
Volume4
Issue number9
DOIs
StatePublished - Sep 11 2009
Externally publishedYes

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astrocytes
Neuralgia
Crosstalk
Spinal Nerves
Astrocytes
Neurons
pain
nerve tissue
Posterior Horn Cells
Ligation
neurons
Wounds and Injuries
Proto-Oncogene Proteins c-fos
Oligodeoxyribonucleotides
Glial Fibrillary Acidic Protein
Hyperalgesia
Chemical activation
neuroglia
Pain
proto-oncogenes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Crosstalk between spinal astrocytes and nuerons in nerve injury-injuced neuropathic pain. / Wang, Wei; Wang, Wen; Mei, Xiaopeng; Huang, Jing; Wei, Yanyan; Wang, Yayun; Wu, Shengxi; Li, Yunqing.

In: PLoS One, Vol. 4, No. 9, e6973, 11.09.2009.

Research output: Contribution to journalArticle

Wang, Wei ; Wang, Wen ; Mei, Xiaopeng ; Huang, Jing ; Wei, Yanyan ; Wang, Yayun ; Wu, Shengxi ; Li, Yunqing. / Crosstalk between spinal astrocytes and nuerons in nerve injury-injuced neuropathic pain. In: PLoS One. 2009 ; Vol. 4, No. 9.
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