Crossed immunoelectrophoretic analysis of antigenic composition of B-subunit/whole-cell and whole-cell only killed oral cholera vaccines

I. Čiẑnár, C. R. Ahsan, A. Rahman, M. Shahabuddin, G. Bartková, J. D. Clemens, D. A. Sack

Research output: Contribution to journalArticle

Abstract

Crossed immunoelectrophoresis was used to identify antigens preserved in the whole-cell component of oral cholera vaccines tested in the field trial in Bangladesh. The composition and immunogenicity of the vaccine antigens were compared with those of antigens obtained from live cells of Vibrio cholerae 01 of both biovars and serovars. The whole-cell component of the vaccine contained ten antigens in comparison with the live Vibrio cells which revealed the presence of 30 antigens. The whole-cell component contained lipopolysaccharide, flagellar antigen, one cell-bound haemagglutinin and at least six outer membrane protein antigens.

Original languageEnglish (US)
Pages (from-to)591-596
Number of pages6
JournalVaccine
Volume10
Issue number9
DOIs
StatePublished - 1992

Keywords

  • B-subunit-whole-cell
  • Cholera
  • antigens
  • crossed immunoelectrophoresis
  • oral vaccine
  • whole-cell only

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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