Cross-reactivity of Shigella flexneri serotype 2a O antigen antibodies following immunization or infection

Lillian L. Van De Verg, Natalie O. Bendiuk, Karen Kotloff, Michelle M. Marsh, Jennifer L. Ruckert, Jill L. Puryear, David N. Taylor, Antoinette B. Hartman, N. O. Oryshkewych

Research output: Contribution to journalArticlepeer-review

Abstract

To study the cross-reactivity pattern of Shigella flexneri 2a O-antigen antibodies, sera from humans and monkeys challenged with S. flexneri 2a, and from humans and guinea pigs immunized with a recombinant vaccine expressing serotype 2a O-antigen, were tested against a panel of lipopolysaccharide extracted from heterologous S. flexneri. Sera from the two groups of humans, who were volunteers in either a clinical challenge or vaccination study, showed similar patterns: cross-reactivity was more often seen with IgA antibodies, and these were mostly cross-reactive with serotype 2b, which shares the type II antigen, and serotypes 1a, 5a, and Y, which share 4 or 3,4 group antigen, with 2a. The majority of sera from immunized guinea pigs showed both IgG and IgA cross-reactivity with 1a, 5a, and Y, but not 2b. The majority of sera from challenged monkeys showed cross-reactivity with almost all flexneri serotypes tested, with 1a, 2b, and Y being recognized most often, and the cross-reactive antibodies were more often IgG than IgA. These results show that either immunization or challenge with the 2a serotype induces cross-reactive antibodies which recognize similar subsets of heterologous serotypes, and suggest that it may be possible to design multivalent vaccines against S. flexneri.

Original languageEnglish (US)
Pages (from-to)1062-1068
Number of pages7
JournalVaccine
Volume14
Issue number11
DOIs
StatePublished - Aug 1996

Keywords

  • Antibodies
  • Cross-reactivity
  • LPS
  • Shigella flexneri 2a
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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