The isotopic antiglobulin test modified for use with tissue culture cellsgrown as adherent monolayers resulted in aquick, sensitive microassay. Serum, prepared from blood of mice hyperimmunizedwith a syngeneic C57BL/6N tumor inducedby 3-methylcholanthrene (MCA) in vitro, cross-reacted with 3 of 7 different MCA-induced tumors. After absorption of serum, the activity was blocked by all MCA-inducedtumor lines and a normal fibroblast line, butnot by normal spleen cells. There was a weakcross-reactivity with murine Moloney andGross virus-induced tumors. A similar weakreactivity with C57BL/6N multiparous exbreeder serum indicated a possible relationship to fetal antigen.
ASJC Scopus subject areas
- Cancer Research