Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses

Christopher W. Pohlmeyer, Sarah B. Laskey, Sarah Beck, Daniel C. Xu, Adam A. Capoferri, Caroline C. Garliss, Megan E. May, Alison Livingston, Walt Lichmira, Richard D Moore, M. Sue Leffell, Nicholas J. Butler, Jennifer Thorne, John A. Flynn, Robert F Siliciano, Joel N Blankson

Research output: Contribution to journalArticle

Abstract

Heterologous immunity is an important aspect of the adaptive immune response. We hypothesized that this process could modulate the HIV-1-specific CD8+ T cell response, which has been shown to play an important role in HIV-1 immunity and control. We found that stimulation of peripheral blood mononuclear cells (PBMCs) from HIV-1-positive subjects with microbial peptides that were cross-reactive with immunodominant HIV-1 epitopes resulted in dramatic expansion of HIV-1-specific CD8+ T cells. Interestingly, the TCR repertoire of HIV-1-specific CD8+ T cells generated by ex vivo stimulation of PBMCs using HIV-1 peptide was different from that of cells stimulated with cross-reactive microbial peptides in some HIV-1-positive subjects. Despite these differences, CD8+ T cells stimulated with either HIV-1 or cross-reactive peptides effectively suppressed HIV-1 replication in autologous CD4+ T cells. These data suggest that exposure to cross-reactive microbial antigens can modulate HIV-1-specific immunity.

Original languageEnglish (US)
Article numbere0192098
JournalPLoS One
Volume13
Issue number2
DOIs
StatePublished - Feb 1 2018

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T-cells
Human immunodeficiency virus 1
HIV-1
T-lymphocytes
HIV
peptides
T-Lymphocytes
Peptides
Blood
immunity
mononuclear leukocytes
Epitopes
Heterologous Immunity
Immunity
Blood Cells
Antigens
Adaptive Immunity
epitopes
antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses. / Pohlmeyer, Christopher W.; Laskey, Sarah B.; Beck, Sarah; Xu, Daniel C.; Capoferri, Adam A.; Garliss, Caroline C.; May, Megan E.; Livingston, Alison; Lichmira, Walt; Moore, Richard D; Sue Leffell, M.; Butler, Nicholas J.; Thorne, Jennifer; Flynn, John A.; Siliciano, Robert F; Blankson, Joel N.

In: PLoS One, Vol. 13, No. 2, e0192098, 01.02.2018.

Research output: Contribution to journalArticle

Pohlmeyer, CW, Laskey, SB, Beck, S, Xu, DC, Capoferri, AA, Garliss, CC, May, ME, Livingston, A, Lichmira, W, Moore, RD, Sue Leffell, M, Butler, NJ, Thorne, J, Flynn, JA, Siliciano, RF & Blankson, JN 2018, 'Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses', PLoS One, vol. 13, no. 2, e0192098. https://doi.org/10.1371/journal.pone.0192098
Pohlmeyer CW, Laskey SB, Beck S, Xu DC, Capoferri AA, Garliss CC et al. Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses. PLoS One. 2018 Feb 1;13(2). e0192098. https://doi.org/10.1371/journal.pone.0192098
Pohlmeyer, Christopher W. ; Laskey, Sarah B. ; Beck, Sarah ; Xu, Daniel C. ; Capoferri, Adam A. ; Garliss, Caroline C. ; May, Megan E. ; Livingston, Alison ; Lichmira, Walt ; Moore, Richard D ; Sue Leffell, M. ; Butler, Nicholas J. ; Thorne, Jennifer ; Flynn, John A. ; Siliciano, Robert F ; Blankson, Joel N. / Cross-reactive microbial peptides can modulate HIV-specific CD8+ T cell responses. In: PLoS One. 2018 ; Vol. 13, No. 2.
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