TY - JOUR
T1 - Cross-modulation by transforming growth factor β in human tuberculosis
T2 - Suppression of antigen-driven blastogenesis and interferon γ production
AU - Hirsch, Christina S.
AU - Hussain, Rabia
AU - Toossi, Zahra
AU - Dawood, Ghaffar
AU - Shahid, Firdaus
AU - Ellner, Jerrold J.
PY - 1996/4/16
Y1 - 1996/4/16
N2 - In tuberculosis, Mycobacterium tuberculosis (MTB)-stimulated T-cell responses are depressed transiently, whereas antibody levels are increased. Lymphoproliferative responses of peripheral blood mononuclear cells (PBMCs) from Pakistani tuberculosis (TB) patients to both mycobacterial and candidal antigens were suppressed by ≃50% when compared to healthy purified protein derivative (PPD)-positive household contacts. Production of interferon γ (IFN-γ) in response to PPD also was depressed by 78%. Stimulation with PPD and the 30-kDa α antigen of MTB (30-kDa antigen) induced greater secretion of transforming growth factor β (TGF-β), but not interleukin 10 (IL-10) or tumor necrosis factor α (TNF-α), by PBMCs from TB patients compared to healthy contacts. The degree of suppression correlated with the duration of treatment; patients treated for 1 month. Neutralizing antibody to TGF-β normalized lymphocyte proliferation in response to PPD, partially restored blastogenesis to candidal antigen, and significantly increased PPD.stimulated production of IFN-γ in TB patients but not in contacts. Neutralizing antibody to IL-10 augmented, but did not normalize, T-cell responses to both PPD and candida in TB patients and candidal antigen in contacts. TGF-β, produced in response to MTB antigens, therefore plays a prominent role in down-regulating potentially protective host effector mechanisms and looms as an important mediator of immunosuppression in TB.
AB - In tuberculosis, Mycobacterium tuberculosis (MTB)-stimulated T-cell responses are depressed transiently, whereas antibody levels are increased. Lymphoproliferative responses of peripheral blood mononuclear cells (PBMCs) from Pakistani tuberculosis (TB) patients to both mycobacterial and candidal antigens were suppressed by ≃50% when compared to healthy purified protein derivative (PPD)-positive household contacts. Production of interferon γ (IFN-γ) in response to PPD also was depressed by 78%. Stimulation with PPD and the 30-kDa α antigen of MTB (30-kDa antigen) induced greater secretion of transforming growth factor β (TGF-β), but not interleukin 10 (IL-10) or tumor necrosis factor α (TNF-α), by PBMCs from TB patients compared to healthy contacts. The degree of suppression correlated with the duration of treatment; patients treated for 1 month. Neutralizing antibody to TGF-β normalized lymphocyte proliferation in response to PPD, partially restored blastogenesis to candidal antigen, and significantly increased PPD.stimulated production of IFN-γ in TB patients but not in contacts. Neutralizing antibody to IL-10 augmented, but did not normalize, T-cell responses to both PPD and candida in TB patients and candidal antigen in contacts. TGF-β, produced in response to MTB antigens, therefore plays a prominent role in down-regulating potentially protective host effector mechanisms and looms as an important mediator of immunosuppression in TB.
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U2 - 10.1073/pnas.93.8.3193
DO - 10.1073/pnas.93.8.3193
M3 - Article
C2 - 8622912
AN - SCOPUS:0029959934
SN - 0027-8424
VL - 93
SP - 3193
EP - 3198
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -