Cross-clade recognition and neutralization by the V3 region from clade C human immunodeficiency virus-1 envelope

Lan Wu, Zhi Yong Yang, Ling Xu, Brent Welcher, Sarah Winfrey, Yiming Shao, John R. Mascola, Gary J. Nabel

Research output: Contribution to journalArticle

Abstract

To understand the cross-reactivity of antibodies directed against variable regions of human immunodeficiency virus-1 (HIV-1) envelope (Env), chimeric immunogens were prepared from different clades with modifications in variable regions, and the resulting neutralizing antibody response was evaluated. The V3-specific neutralization activity induced by a clade B immunogen was limited to clade B viruses and was blocked by a clade B V3 peptide, but not by analogous clade A or C V3 peptides. In contrast, the V3 response elicited by a clade C immunogen cross-reacted with sensitive clade B viruses. The V3 region from a clade C virus, when introduced into a clade B sequence, elicited cross-clade activity, which could be reversed by V3 peptides derived from clades A and C. Thus, the anti-V3 antibody response elicited by a clade C immunogen could cross-react with heterologous clade viruses. Additionally, we describe a V1-specific immune response that mediated neutralization limited to the homologous HIV IIIB isolate and may be partially responsible for the commonly observed strain-specific neutralization responses elicited by vaccine immunogens.

Original languageEnglish (US)
Pages (from-to)4995-5002
Number of pages8
JournalVaccine
Volume24
Issue number23
DOIs
StatePublished - Jun 5 2006
Externally publishedYes

Keywords

  • Envelope
  • HIV-1
  • Neutralizing antibodies

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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  • Cite this

    Wu, L., Yang, Z. Y., Xu, L., Welcher, B., Winfrey, S., Shao, Y., Mascola, J. R., & Nabel, G. J. (2006). Cross-clade recognition and neutralization by the V3 region from clade C human immunodeficiency virus-1 envelope. Vaccine, 24(23), 4995-5002. https://doi.org/10.1016/j.vaccine.2006.03.083