Cronobacter spp. (previously Enterobacter sakazakii) invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells

Chandrakant P. Giri, Kensuke Shima, Ben D. Tall, Sherill Curtis, Venugopal Sathyamoorthy, Brock Hanisch, Kwang Sik Kim, Dennis J. Kopecko

Research output: Contribution to journalArticle

Abstract

The mechanism of Cronobacter pathogenesis in neonatal meningitis and potential virulence factors (aside from host cell invasion ability) remain largely unknown. To ascertain whether Cronobacter can invade and transcytose across intestinal epithelial cells, enter into the blood stream and then transcytose across the blood-brain-barrier, we have utilized human intestinal INT407 and Caco-2 cells and brain microvascular endothelial cell (HBMEC) monolayers on Transwell filters as experimental model systems. Our data indicate a wide range of heterogeneity with respect to invasion efficiency among twenty-three Cronobacter isolates screened. For selected isolates, we observed significant levels of transcytosis for Cronobacter sakazakii across tight monolayers of both Caco-2 and HBMEC, mimicking invivo ability to cross the intestine as well as the blood brain barrier, and at a frequency equivalent to that of a control meningitis-causing Escherichia coli K1 strain. Finally, EM analysis demonstrated intracellular Cronobacter bacteria within host vacuoles in HBMEC, as well as transcytosed bacteria at the basolateral surface. These data reveal that certain Cronobacter isolates can invade and translocate across both cultured human intestinal epithelial cells and HBMEC, thus demonstrating a potential path for neonatal infections of the central nervous system (CNS) following oral ingestion.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalMicrobial Pathogenesis
Volume52
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Cronobacter
Cronobacter sakazakii
Endothelial Cells
Epithelial Cells
Brain
Blood-Brain Barrier
Escherichia coli Meningitis
Bacteria
Transcytosis
Central Nervous System Infections
Caco-2 Cells
Virulence Factors
Vacuoles
Meningitis
Intestines
Theoretical Models
Eating

Keywords

  • Blood-brain barrier
  • CNS
  • Cronobacter sakazakii
  • Meningitis
  • Transcytosis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Cronobacter spp. (previously Enterobacter sakazakii) invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells. / Giri, Chandrakant P.; Shima, Kensuke; Tall, Ben D.; Curtis, Sherill; Sathyamoorthy, Venugopal; Hanisch, Brock; Kim, Kwang Sik; Kopecko, Dennis J.

In: Microbial Pathogenesis, Vol. 52, No. 2, 02.2012, p. 140-147.

Research output: Contribution to journalArticle

Giri, Chandrakant P. ; Shima, Kensuke ; Tall, Ben D. ; Curtis, Sherill ; Sathyamoorthy, Venugopal ; Hanisch, Brock ; Kim, Kwang Sik ; Kopecko, Dennis J. / Cronobacter spp. (previously Enterobacter sakazakii) invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells. In: Microbial Pathogenesis. 2012 ; Vol. 52, No. 2. pp. 140-147.
@article{39350274bb084ef7a5458e86ff241602,
title = "Cronobacter spp. (previously Enterobacter sakazakii) invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells",
abstract = "The mechanism of Cronobacter pathogenesis in neonatal meningitis and potential virulence factors (aside from host cell invasion ability) remain largely unknown. To ascertain whether Cronobacter can invade and transcytose across intestinal epithelial cells, enter into the blood stream and then transcytose across the blood-brain-barrier, we have utilized human intestinal INT407 and Caco-2 cells and brain microvascular endothelial cell (HBMEC) monolayers on Transwell filters as experimental model systems. Our data indicate a wide range of heterogeneity with respect to invasion efficiency among twenty-three Cronobacter isolates screened. For selected isolates, we observed significant levels of transcytosis for Cronobacter sakazakii across tight monolayers of both Caco-2 and HBMEC, mimicking invivo ability to cross the intestine as well as the blood brain barrier, and at a frequency equivalent to that of a control meningitis-causing Escherichia coli K1 strain. Finally, EM analysis demonstrated intracellular Cronobacter bacteria within host vacuoles in HBMEC, as well as transcytosed bacteria at the basolateral surface. These data reveal that certain Cronobacter isolates can invade and translocate across both cultured human intestinal epithelial cells and HBMEC, thus demonstrating a potential path for neonatal infections of the central nervous system (CNS) following oral ingestion.",
keywords = "Blood-brain barrier, CNS, Cronobacter sakazakii, Meningitis, Transcytosis",
author = "Giri, {Chandrakant P.} and Kensuke Shima and Tall, {Ben D.} and Sherill Curtis and Venugopal Sathyamoorthy and Brock Hanisch and Kim, {Kwang Sik} and Kopecko, {Dennis J.}",
year = "2012",
month = "2",
doi = "10.1016/j.micpath.2011.10.003",
language = "English (US)",
volume = "52",
pages = "140--147",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Cronobacter spp. (previously Enterobacter sakazakii) invade and translocate across both cultured human intestinal epithelial cells and human brain microvascular endothelial cells

AU - Giri, Chandrakant P.

AU - Shima, Kensuke

AU - Tall, Ben D.

AU - Curtis, Sherill

AU - Sathyamoorthy, Venugopal

AU - Hanisch, Brock

AU - Kim, Kwang Sik

AU - Kopecko, Dennis J.

PY - 2012/2

Y1 - 2012/2

N2 - The mechanism of Cronobacter pathogenesis in neonatal meningitis and potential virulence factors (aside from host cell invasion ability) remain largely unknown. To ascertain whether Cronobacter can invade and transcytose across intestinal epithelial cells, enter into the blood stream and then transcytose across the blood-brain-barrier, we have utilized human intestinal INT407 and Caco-2 cells and brain microvascular endothelial cell (HBMEC) monolayers on Transwell filters as experimental model systems. Our data indicate a wide range of heterogeneity with respect to invasion efficiency among twenty-three Cronobacter isolates screened. For selected isolates, we observed significant levels of transcytosis for Cronobacter sakazakii across tight monolayers of both Caco-2 and HBMEC, mimicking invivo ability to cross the intestine as well as the blood brain barrier, and at a frequency equivalent to that of a control meningitis-causing Escherichia coli K1 strain. Finally, EM analysis demonstrated intracellular Cronobacter bacteria within host vacuoles in HBMEC, as well as transcytosed bacteria at the basolateral surface. These data reveal that certain Cronobacter isolates can invade and translocate across both cultured human intestinal epithelial cells and HBMEC, thus demonstrating a potential path for neonatal infections of the central nervous system (CNS) following oral ingestion.

AB - The mechanism of Cronobacter pathogenesis in neonatal meningitis and potential virulence factors (aside from host cell invasion ability) remain largely unknown. To ascertain whether Cronobacter can invade and transcytose across intestinal epithelial cells, enter into the blood stream and then transcytose across the blood-brain-barrier, we have utilized human intestinal INT407 and Caco-2 cells and brain microvascular endothelial cell (HBMEC) monolayers on Transwell filters as experimental model systems. Our data indicate a wide range of heterogeneity with respect to invasion efficiency among twenty-three Cronobacter isolates screened. For selected isolates, we observed significant levels of transcytosis for Cronobacter sakazakii across tight monolayers of both Caco-2 and HBMEC, mimicking invivo ability to cross the intestine as well as the blood brain barrier, and at a frequency equivalent to that of a control meningitis-causing Escherichia coli K1 strain. Finally, EM analysis demonstrated intracellular Cronobacter bacteria within host vacuoles in HBMEC, as well as transcytosed bacteria at the basolateral surface. These data reveal that certain Cronobacter isolates can invade and translocate across both cultured human intestinal epithelial cells and HBMEC, thus demonstrating a potential path for neonatal infections of the central nervous system (CNS) following oral ingestion.

KW - Blood-brain barrier

KW - CNS

KW - Cronobacter sakazakii

KW - Meningitis

KW - Transcytosis

UR - http://www.scopus.com/inward/record.url?scp=84855187711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855187711&partnerID=8YFLogxK

U2 - 10.1016/j.micpath.2011.10.003

DO - 10.1016/j.micpath.2011.10.003

M3 - Article

VL - 52

SP - 140

EP - 147

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

IS - 2

ER -