Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development

Zhiyu Huang, Umar Yazdani, Katherine L. Thompson-Peer, Alex L Kolodkin, Jonathan R. Terman

Research output: Contribution to journalArticle

Abstract

Members of the Cas family of Src homology 3 (SH3)-domain-containing cytosolic signaling proteins are crucial regulators of actin cytoskeletal dynamics in non-neuronal cells; however, their neuronal functions are poorly understood. Here, we identify a Drosophila Cas (DCas), find that Cas proteins are highly expressed in neurons and show that DCas is required for correct axon guidance during development. Functional analyses reveal that Cas specifies axon guidance by regulating the degree of fasciculation among axons. These guidance defects are similar to those observed in integrin mutants, and genetic analysis shows that integrins function together with Cas to facilitate axonal defasciculation. These results strongly support Cas proteins working together with integrins in vivo to direct axon guidance events.

Original languageEnglish (US)
Pages (from-to)2337-2347
Number of pages11
JournalDevelopment
Volume134
Issue number12
DOIs
StatePublished - Jun 2007

Fingerprint

Crk-Associated Substrate Protein
Integrins
Drosophila
src Homology Domains
Axon Guidance
Actins
Neurons

Keywords

  • Axon guidance
  • Casi
  • Drosophila
  • p130Cas

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development. / Huang, Zhiyu; Yazdani, Umar; Thompson-Peer, Katherine L.; Kolodkin, Alex L; Terman, Jonathan R.

In: Development, Vol. 134, No. 12, 06.2007, p. 2337-2347.

Research output: Contribution to journalArticle

Huang, Zhiyu ; Yazdani, Umar ; Thompson-Peer, Katherine L. ; Kolodkin, Alex L ; Terman, Jonathan R. / Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development. In: Development. 2007 ; Vol. 134, No. 12. pp. 2337-2347.
@article{0685edf748b14f19b337b3357afe1a35,
title = "Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development",
abstract = "Members of the Cas family of Src homology 3 (SH3)-domain-containing cytosolic signaling proteins are crucial regulators of actin cytoskeletal dynamics in non-neuronal cells; however, their neuronal functions are poorly understood. Here, we identify a Drosophila Cas (DCas), find that Cas proteins are highly expressed in neurons and show that DCas is required for correct axon guidance during development. Functional analyses reveal that Cas specifies axon guidance by regulating the degree of fasciculation among axons. These guidance defects are similar to those observed in integrin mutants, and genetic analysis shows that integrins function together with Cas to facilitate axonal defasciculation. These results strongly support Cas proteins working together with integrins in vivo to direct axon guidance events.",
keywords = "Axon guidance, Casi, Drosophila, p130Cas",
author = "Zhiyu Huang and Umar Yazdani and Thompson-Peer, {Katherine L.} and Kolodkin, {Alex L} and Terman, {Jonathan R.}",
year = "2007",
month = "6",
doi = "10.1242/dev.004242",
language = "English (US)",
volume = "134",
pages = "2337--2347",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "12",

}

TY - JOUR

T1 - Crk-associated substrate (Cas) signaling protein functions with integrins to specify axon guidance during development

AU - Huang, Zhiyu

AU - Yazdani, Umar

AU - Thompson-Peer, Katherine L.

AU - Kolodkin, Alex L

AU - Terman, Jonathan R.

PY - 2007/6

Y1 - 2007/6

N2 - Members of the Cas family of Src homology 3 (SH3)-domain-containing cytosolic signaling proteins are crucial regulators of actin cytoskeletal dynamics in non-neuronal cells; however, their neuronal functions are poorly understood. Here, we identify a Drosophila Cas (DCas), find that Cas proteins are highly expressed in neurons and show that DCas is required for correct axon guidance during development. Functional analyses reveal that Cas specifies axon guidance by regulating the degree of fasciculation among axons. These guidance defects are similar to those observed in integrin mutants, and genetic analysis shows that integrins function together with Cas to facilitate axonal defasciculation. These results strongly support Cas proteins working together with integrins in vivo to direct axon guidance events.

AB - Members of the Cas family of Src homology 3 (SH3)-domain-containing cytosolic signaling proteins are crucial regulators of actin cytoskeletal dynamics in non-neuronal cells; however, their neuronal functions are poorly understood. Here, we identify a Drosophila Cas (DCas), find that Cas proteins are highly expressed in neurons and show that DCas is required for correct axon guidance during development. Functional analyses reveal that Cas specifies axon guidance by regulating the degree of fasciculation among axons. These guidance defects are similar to those observed in integrin mutants, and genetic analysis shows that integrins function together with Cas to facilitate axonal defasciculation. These results strongly support Cas proteins working together with integrins in vivo to direct axon guidance events.

KW - Axon guidance

KW - Casi

KW - Drosophila

KW - p130Cas

UR - http://www.scopus.com/inward/record.url?scp=34347327207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34347327207&partnerID=8YFLogxK

U2 - 10.1242/dev.004242

DO - 10.1242/dev.004242

M3 - Article

C2 - 17537798

AN - SCOPUS:34347327207

VL - 134

SP - 2337

EP - 2347

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 12

ER -