TY - JOUR
T1 - Critical Review - Approaches for the Electrochemical Interrogation of DNA-Based Sensors
T2 - A Critical Review
AU - Pellitero, Miguel Aller
AU - Shaver, Alexander
AU - Arroyo-Currás, Netzahualcóyotl
N1 - Publisher Copyright:
© The Author(s) 2019. Published by ECS.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - The desire to improve and decentralize diagnostic platforms to facilitate highly precise and personalized medicine has motivated the development of a large number of electrochemical sensing technologies. Such a development has been facilitated by electrochemistry's unparalleled ability to achieve highly specific molecular measurements in complex biological fluids, without the need for expensive instrumentation. However, for decades, progress in the field had been constrained to systems that depended on the chemical reactivity of the analyte, obstructing the generalizability of such platforms beyond redox- or enzymatically active clinical targets. Thus, the pursuit of alternative, more general strategies, coupled to the timely technological advances in DNA sequencing, led to the development of DNA-based electrochemical sensors. The analytical value of these arises from the structural customizability of DNA and its ability to bind analytes ranging from ions and small molecules to whole proteins and cells. This versatility extends to interrogation methods, as DNA-based sensors work through a variety of detection schemes that can be probed via many electroanalytical techniques. As a reference for those experienced in the field, and to guide the unexperienced scientist, here we review the specific advantages of the electroanalytical methods most commonly used for the interrogation of DNA-based sensors.
AB - The desire to improve and decentralize diagnostic platforms to facilitate highly precise and personalized medicine has motivated the development of a large number of electrochemical sensing technologies. Such a development has been facilitated by electrochemistry's unparalleled ability to achieve highly specific molecular measurements in complex biological fluids, without the need for expensive instrumentation. However, for decades, progress in the field had been constrained to systems that depended on the chemical reactivity of the analyte, obstructing the generalizability of such platforms beyond redox- or enzymatically active clinical targets. Thus, the pursuit of alternative, more general strategies, coupled to the timely technological advances in DNA sequencing, led to the development of DNA-based electrochemical sensors. The analytical value of these arises from the structural customizability of DNA and its ability to bind analytes ranging from ions and small molecules to whole proteins and cells. This versatility extends to interrogation methods, as DNA-based sensors work through a variety of detection schemes that can be probed via many electroanalytical techniques. As a reference for those experienced in the field, and to guide the unexperienced scientist, here we review the specific advantages of the electroanalytical methods most commonly used for the interrogation of DNA-based sensors.
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U2 - 10.1149/2.0292003JES
DO - 10.1149/2.0292003JES
M3 - Review article
AN - SCOPUS:85081166956
VL - 167
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
SN - 0013-4651
IS - 3
M1 - 037529
ER -