Critical mixing in monolayer mixtures of phospholipid and cholesterol

Sriram Subramaniam, Harden M. McConnell

Research output: Contribution to journalArticle

Abstract

We report the observation of two-dimensional fluid-fluid immiscibility in monolayer mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol spread at an air-water interface. Coexisting fluid domains were visualized by fluorescence microscopy in monolayers that were stained with low concentrations of a fluorescent lipid probe. Monolayers with cholesterol concentrations in the range 0-30 mol % show reversible two-phase to one-phase transformations at characteristic densities and surface pressures. For cholesterol concentrations near 30 mol %, this isothermal transition is characterized by fluctuations in the shape of the domain boundaries, indicating the presence of a critical point at this composition.

Original languageEnglish (US)
Pages (from-to)1715-1718
Number of pages4
JournalJournal of Physical Chemistry
Volume91
Issue number7
StatePublished - 1987
Externally publishedYes

Fingerprint

Cholesterol
Phospholipids
cholesterol
Monolayers
Fluids
fluids
Dimyristoylphosphatidylcholine
Fluorescence microscopy
Lipids
phase transformations
lipids
low concentrations
critical point
solubility
Solubility
Phase transitions
microscopy
fluorescence
Water
probes

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Subramaniam, S., & McConnell, H. M. (1987). Critical mixing in monolayer mixtures of phospholipid and cholesterol. Journal of Physical Chemistry, 91(7), 1715-1718.

Critical mixing in monolayer mixtures of phospholipid and cholesterol. / Subramaniam, Sriram; McConnell, Harden M.

In: Journal of Physical Chemistry, Vol. 91, No. 7, 1987, p. 1715-1718.

Research output: Contribution to journalArticle

Subramaniam, S & McConnell, HM 1987, 'Critical mixing in monolayer mixtures of phospholipid and cholesterol', Journal of Physical Chemistry, vol. 91, no. 7, pp. 1715-1718.
Subramaniam, Sriram ; McConnell, Harden M. / Critical mixing in monolayer mixtures of phospholipid and cholesterol. In: Journal of Physical Chemistry. 1987 ; Vol. 91, No. 7. pp. 1715-1718.
@article{1ffdc97bb3be4da89b900ebfc32037d0,
title = "Critical mixing in monolayer mixtures of phospholipid and cholesterol",
abstract = "We report the observation of two-dimensional fluid-fluid immiscibility in monolayer mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol spread at an air-water interface. Coexisting fluid domains were visualized by fluorescence microscopy in monolayers that were stained with low concentrations of a fluorescent lipid probe. Monolayers with cholesterol concentrations in the range 0-30 mol {\%} show reversible two-phase to one-phase transformations at characteristic densities and surface pressures. For cholesterol concentrations near 30 mol {\%}, this isothermal transition is characterized by fluctuations in the shape of the domain boundaries, indicating the presence of a critical point at this composition.",
author = "Sriram Subramaniam and McConnell, {Harden M.}",
year = "1987",
language = "English (US)",
volume = "91",
pages = "1715--1718",
journal = "Journal of Physical Chemistry",
issn = "0022-3654",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Critical mixing in monolayer mixtures of phospholipid and cholesterol

AU - Subramaniam, Sriram

AU - McConnell, Harden M.

PY - 1987

Y1 - 1987

N2 - We report the observation of two-dimensional fluid-fluid immiscibility in monolayer mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol spread at an air-water interface. Coexisting fluid domains were visualized by fluorescence microscopy in monolayers that were stained with low concentrations of a fluorescent lipid probe. Monolayers with cholesterol concentrations in the range 0-30 mol % show reversible two-phase to one-phase transformations at characteristic densities and surface pressures. For cholesterol concentrations near 30 mol %, this isothermal transition is characterized by fluctuations in the shape of the domain boundaries, indicating the presence of a critical point at this composition.

AB - We report the observation of two-dimensional fluid-fluid immiscibility in monolayer mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol spread at an air-water interface. Coexisting fluid domains were visualized by fluorescence microscopy in monolayers that were stained with low concentrations of a fluorescent lipid probe. Monolayers with cholesterol concentrations in the range 0-30 mol % show reversible two-phase to one-phase transformations at characteristic densities and surface pressures. For cholesterol concentrations near 30 mol %, this isothermal transition is characterized by fluctuations in the shape of the domain boundaries, indicating the presence of a critical point at this composition.

UR - http://www.scopus.com/inward/record.url?scp=33845281464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845281464&partnerID=8YFLogxK

M3 - Article

VL - 91

SP - 1715

EP - 1718

JO - Journal of Physical Chemistry

JF - Journal of Physical Chemistry

SN - 0022-3654

IS - 7

ER -