Creation of a bacterial cell controlled by a chemically synthesized genome

Daniel G. Gibson, John I. Glass, Carole Lartigue, Vladimir N. Noskov, Ray Yuan Chuang, Mikkel A. Algire, Gwynedd A. Benders, Michael Montague, Li Ma, Monzia M. Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A. Denisova, Lei Young, Zhi Ng Qi, Thomas H. Segall-Shapiro, Christopher H. CalveyPrashanth P. Parmar, Clyde A. Hutchison, Hamilton O. Smith, J. Craig Venter

Research output: Contribution to journalArticle

Abstract

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

Original languageEnglish (US)
Pages (from-to)52-56
Number of pages5
JournalScience
Volume329
Issue number5987
DOIs
StatePublished - Jul 2 2010
Externally publishedYes

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Mycoplasma mycoides
Genome
Sequence Deletion
Gene Deletion
Base Pairing
Chromosomes
Transplantation
DNA

ASJC Scopus subject areas

  • General

Cite this

Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R. Y., Algire, M. A., ... Venter, J. C. (2010). Creation of a bacterial cell controlled by a chemically synthesized genome. Science, 329(5987), 52-56. https://doi.org/10.1126/science.1190719

Creation of a bacterial cell controlled by a chemically synthesized genome. / Gibson, Daniel G.; Glass, John I.; Lartigue, Carole; Noskov, Vladimir N.; Chuang, Ray Yuan; Algire, Mikkel A.; Benders, Gwynedd A.; Montague, Michael; Ma, Li; Moodie, Monzia M.; Merryman, Chuck; Vashee, Sanjay; Krishnakumar, Radha; Assad-Garcia, Nacyra; Andrews-Pfannkoch, Cynthia; Denisova, Evgeniya A.; Young, Lei; Qi, Zhi Ng; Segall-Shapiro, Thomas H.; Calvey, Christopher H.; Parmar, Prashanth P.; Hutchison, Clyde A.; Smith, Hamilton O.; Venter, J. Craig.

In: Science, Vol. 329, No. 5987, 02.07.2010, p. 52-56.

Research output: Contribution to journalArticle

Gibson, DG, Glass, JI, Lartigue, C, Noskov, VN, Chuang, RY, Algire, MA, Benders, GA, Montague, M, Ma, L, Moodie, MM, Merryman, C, Vashee, S, Krishnakumar, R, Assad-Garcia, N, Andrews-Pfannkoch, C, Denisova, EA, Young, L, Qi, ZN, Segall-Shapiro, TH, Calvey, CH, Parmar, PP, Hutchison, CA, Smith, HO & Venter, JC 2010, 'Creation of a bacterial cell controlled by a chemically synthesized genome', Science, vol. 329, no. 5987, pp. 52-56. https://doi.org/10.1126/science.1190719
Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA et al. Creation of a bacterial cell controlled by a chemically synthesized genome. Science. 2010 Jul 2;329(5987):52-56. https://doi.org/10.1126/science.1190719
Gibson, Daniel G. ; Glass, John I. ; Lartigue, Carole ; Noskov, Vladimir N. ; Chuang, Ray Yuan ; Algire, Mikkel A. ; Benders, Gwynedd A. ; Montague, Michael ; Ma, Li ; Moodie, Monzia M. ; Merryman, Chuck ; Vashee, Sanjay ; Krishnakumar, Radha ; Assad-Garcia, Nacyra ; Andrews-Pfannkoch, Cynthia ; Denisova, Evgeniya A. ; Young, Lei ; Qi, Zhi Ng ; Segall-Shapiro, Thomas H. ; Calvey, Christopher H. ; Parmar, Prashanth P. ; Hutchison, Clyde A. ; Smith, Hamilton O. ; Venter, J. Craig. / Creation of a bacterial cell controlled by a chemically synthesized genome. In: Science. 2010 ; Vol. 329, No. 5987. pp. 52-56.
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