Creation of a bacterial cell controlled by a chemically synthesized genome

Daniel G. Gibson, John I. Glass, Carole Lartigue, Vladimir N. Noskov, Ray Yuan Chuang, Mikkel A. Algire, Gwynedd A. Benders, Michael G. Montague, Li Ma, Monzia M. Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A. Denisova, Lei Young, Zhi Ng Qi, Thomas H. Segall-Shapiro, Christopher H. CalveyPrashanth P. Parmar, Clyde A. Hutchison, Hamilton O. Smith, J. Craig Venter

Research output: Contribution to journalArticlepeer-review

Abstract

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

Original languageEnglish (US)
Pages (from-to)52-56
Number of pages5
JournalScience
Volume329
Issue number5987
DOIs
StatePublished - Jul 2 2010

ASJC Scopus subject areas

  • General

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