Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching

Shuming Zhang, Xi Liu, Sebastian F. Barreto-Ortiz, Yixuan Yu, Brian P. Ginn, Nicholas A. DeSantis, Daphne L. Hutton, Warren L Grayson, Fu Zhai Cui, Brian A. Korgel, Sharon Gerecht, Hai Quan Mao

Research output: Contribution to journalArticle

Abstract

Hydrogels have been widely used for 3-dimensional (3D) cell culture and tissue regeneration due to their tunable biochemical and physicochemical properties as well as their high water content, which resembles the aqueous microenvironment of the natural extracellular matrix. While many properties of natural hydrogel matrices are modifiable, their intrinsic isotropic structure limits the control over cellular organization, which is critical to restore tissue function. Here we report a generic approach to incorporate alignment topography inside the hydrogel matrix using a combination of electrical and mechanical stretching. Hydrogel fibres with uniaxial alignment were prepared from aqueous solutions of natural polymers such as alginate, fibrin, gelatin, and hyaluronic acid under ambient conditions. The unique internal alignment feature drastically enhances the mechanical properties of the hydrogel microfibres. Furthermore, the facile, organic solvent-free processing conditions are amenable to the incorporation of live cells within the hydrogel fibre or on the fibre surface; both approaches effectively induce cellular alignment. This work demonstrates a versatile and scalable strategy to create aligned hydrogel microfibres from various natural polymers.

Original languageEnglish (US)
Pages (from-to)3243-3251
Number of pages9
JournalBiomaterials
Volume35
Issue number10
DOIs
StatePublished - Mar 2014

Fingerprint

Hydrogel
Hydrogels
Stretching
Polymers
Natural polymers
Fibers
Tissue regeneration
Hyaluronic acid
Hyaluronic Acid
Gelatin
Fibrin
Cell culture
Alginate
Organic solvents
Topography
Water content
Extracellular Matrix
Regeneration
Cell Culture Techniques
Tissue

Keywords

  • Alginate
  • Fibrin
  • Hyaluronic acid
  • Hydrogel
  • Microstructure

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Zhang, S., Liu, X., Barreto-Ortiz, S. F., Yu, Y., Ginn, B. P., DeSantis, N. A., ... Mao, H. Q. (2014). Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching. Biomaterials, 35(10), 3243-3251. https://doi.org/10.1016/j.biomaterials.2013.12.081

Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching. / Zhang, Shuming; Liu, Xi; Barreto-Ortiz, Sebastian F.; Yu, Yixuan; Ginn, Brian P.; DeSantis, Nicholas A.; Hutton, Daphne L.; Grayson, Warren L; Cui, Fu Zhai; Korgel, Brian A.; Gerecht, Sharon; Mao, Hai Quan.

In: Biomaterials, Vol. 35, No. 10, 03.2014, p. 3243-3251.

Research output: Contribution to journalArticle

Zhang, S, Liu, X, Barreto-Ortiz, SF, Yu, Y, Ginn, BP, DeSantis, NA, Hutton, DL, Grayson, WL, Cui, FZ, Korgel, BA, Gerecht, S & Mao, HQ 2014, 'Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching', Biomaterials, vol. 35, no. 10, pp. 3243-3251. https://doi.org/10.1016/j.biomaterials.2013.12.081
Zhang, Shuming ; Liu, Xi ; Barreto-Ortiz, Sebastian F. ; Yu, Yixuan ; Ginn, Brian P. ; DeSantis, Nicholas A. ; Hutton, Daphne L. ; Grayson, Warren L ; Cui, Fu Zhai ; Korgel, Brian A. ; Gerecht, Sharon ; Mao, Hai Quan. / Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching. In: Biomaterials. 2014 ; Vol. 35, No. 10. pp. 3243-3251.
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