Creating new fluorescent probes for cell biology

Jin Zhang; Robert E. Campbell; Alice Y. Ting; Roger Y. Tsien

doi:10.1038/nrm976

Creating new fluorescent probes for cell biology

Jin Zhang, Robert E. Campbell, Alice Y. Ting, Roger Y. Tsien

Research output: Contribution to journal › Article › peer-review

1612 Scopus citations

Abstract

Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

Original language	English (US)
Pages (from-to)	906-918
Number of pages	13
Journal	Nature Reviews Molecular Cell Biology
Volume	3
Issue number	12
DOIs	https://doi.org/10.1038/nrm976
State	Published - Dec 1 2002
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1038/nrm976

Cite this

@article{6aa6caa2207349eba76c838ab13cb588,

title = "Creating new fluorescent probes for cell biology",

abstract = "Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.",

author = "Jin Zhang and Campbell, {Robert E.} and Ting, {Alice Y.} and Tsien, {Roger Y.}",

year = "2002",

month = dec,

day = "1",

doi = "10.1038/nrm976",

language = "English (US)",

volume = "3",

pages = "906--918",

journal = "Nature Reviews Molecular Cell Biology",

issn = "1471-0072",

publisher = "Nature Publishing Group",

number = "12",

}

TY - JOUR

T1 - Creating new fluorescent probes for cell biology

AU - Zhang, Jin

AU - Campbell, Robert E.

AU - Ting, Alice Y.

AU - Tsien, Roger Y.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

AB - Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

UR - http://www.scopus.com/inward/record.url?scp=0036902813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036902813&partnerID=8YFLogxK

U2 - 10.1038/nrm976

DO - 10.1038/nrm976

M3 - Article

C2 - 12461557

AN - SCOPUS:0036902813

SN - 1471-0072

VL - 3

SP - 906

EP - 918

JO - Nature Reviews Molecular Cell Biology

JF - Nature Reviews Molecular Cell Biology

IS - 12

ER -

Creating new fluorescent probes for cell biology

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this