Creatine and phosphocreatine mapping of mouse skeletal muscle by a polynomial and Lorentzian line-shape fitting CEST method

Research output: Contribution to journalArticle

Abstract

Purpose: To obtain high-resolution Cr and PCr maps of mouse skeletal muscle using a polynomial and Lorentzian line-shape fitting (PLOF) CEST method. Methods: Wild-type mice and guanidinoacetate N-methyltransferase–deficient (GAMT-/-) mice that have low Cr and PCr concentrations in muscle were used to assign the Cr and PCr peaks in the Z-spectrum at 11.7 T. A PLOF method was proposed to simultaneously extract and quantify the Cr and PCr by assuming a polynomial function for the background and 2 Lorentzian functions for the CEST peaks at 1.95 ppm and 2.5 ppm. Results: The Z-spectra of phantoms revealed that PCr has 2 CEST peaks (2 ppm and 2.5 ppm), whereas Cr only showed 1 peak at 2 ppm. Comparison of the Z-spectra of wild-type and GAMT-/- mice indicated that, contrary to brain, there was no visible protein guanidinium peak in the skeletal-muscle Z-spectrum, which allowed us to extract clean PCr and Cr CEST signals. High-resolution PCr and Cr concentration maps of mouse skeletal muscle were obtained by the PLOF CEST method after calibration with in vivo MRS. Conclusions: The PLOF method provides an efficient way to map Cr and PCr concentrations simultaneously in the skeletal muscle at high MRI field.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalMagnetic resonance in medicine
Volume81
Issue number1
DOIs
StatePublished - Jan 2019

Keywords

  • chemical exchange saturation transfer
  • creatine
  • guanidinoacetate
  • guanidinoacetate N-methyltransferase deficiency mouse
  • magnetization transfer contrast
  • phosphate guanidinoacetate
  • phosphocreatine
  • polynomial and Lorentzian line-shape fitting

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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