Cranberry juice extract, a mild prooxidant with cytotoxic properties independent of reactive oxygen species

H. Babich, I. M. Ickow, J. H. Weisburg, H. L. Zuckerbraun, A. G. Schuck

Research output: Contribution to journalArticle

Abstract

A cranberry juice extract (CJE), rich in proanthocyanidins, had weak prooxidant properties, generating low levels of hydrogen peroxide (H 2O2) and superoxide. Generation of H2O 2 was pH dependent, increasing at alkaline pH, and was lowered in the presence of catalase and, to a lesser extent, of superoxide dismutase (SOD). Growth inhibition and cytotoxicity were noted towards human oral carcinoma HSC-2 cells, with midpoint cytotoxicity at 200 μg/mL CJE, but not towards human gingival HF-1 fibroblasts. Being a mild prooxidant, CJE toxicity was unaffected by exogenous catalase and pyruvate, scavengers of H2O2, but triggered intracellular synthesis of reduced glutathione, as confirmed by cell staining with Cell Tracker™ Green. The presence of exogenous SOD potentiated the toxicity of CJE, possibly by stabilizing the CJE phenols and hindering their degradative autooxidation. Conversely, 'spent' CJE, i.e. CJE added to cell culture medium and incubated for 24 h at 37 °C prior to use, was much less toxic to HSC-2 cells than was freshly prepared CJE. These differences in toxicity between SOD-stabilized CJE, freshly prepared CJE, and 'spent' CJE were confirmed in HSC-2 cells stained with aceto-orcein, which also indicated that the mode of cell death was by the induction of apoptosis.

Original languageEnglish (US)
Pages (from-to)1358-1365
Number of pages8
JournalPhytotherapy Research
Volume26
Issue number9
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Keywords

  • cranberry juice
  • cytotoxicity
  • experimental
  • phenol
  • prooxidant

ASJC Scopus subject areas

  • Pharmacology

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